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dc.contributor.author
Calimag Williams, Korina
dc.contributor.author
Goicoechea, Hector Casimiro
dc.contributor.author
Campiglia, Andres D.
dc.date.available
2019-04-11T21:55:59Z
dc.date.issued
2011-09
dc.identifier.citation
Calimag Williams, Korina; Goicoechea, Hector Casimiro; Campiglia, Andres D.; Room-temperature fluorescence spectroscopy of monohydroxy metabolites of polycyclic aromatic hydrocarbons on octadecyl extraction membranes; Elsevier Science; Talanta; 85; 4; 9-2011; 1805-1811
dc.identifier.issn
0039-9140
dc.identifier.uri
http://hdl.handle.net/11336/74181
dc.description.abstract
Urine analysis of monohydroxy metabolites is recognized as an accurate assessment of human exposure to polycyclic aromatic hydrocarbons. Despite the sophisticated arsenal of analytical tools, monitoring of monohydroxy metabolites via simple, cost effective and direct methods of analysis still remains a challenge. This article evaluates the analytical potential of solid-phase extraction room-temperature fluorescence spectroscopy for the problem at hand. Extraction membranes serve the dual purpose of sample pre-concentration and solid substrate for RTF measurements. The potential of our proposition is demonstrated with the analysis of 2-hydroxy-fluorene, 1-hydroxy-pyrene, 3-hydroxy-benzo[a]pyrene and 9-hydroxy-phenanthrene in synthetic urine samples. Signal reproducibility is improved with the aid of a sample holder specifically designed for the manual optimization of luminescence signals. Background correction of solid substrates is carried out with the aid of Asymmetric Least Squares. Recovery values for the studied metabolites varied from 99.0 ± 1.2% (3-hydroxy-benzo[a]pyrene) to 99.9 ± 0.05% (1-hydroxy-pyrene). With only 10 mL of urine sample, the limits of detection varied from 57 pg mL -1 (2-hydroxy-fluorene) to 2 pg mL-1 (1-hydroxy-pyrene). Additional figures of merit include a simple experimental procedure for routine screening of numerous samples and compatibility with portable instrumentation for field analysis. Because of the non-destructive nature of fluorescence measurements, membranes can be brought to the lab for subsequent elution and confirmation of compounds via high-resolution techniques.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Asymmetric Least Squares
dc.subject
Monohydroxy Metabolites
dc.subject
Polycyclic Aromatic Hydrocarbons
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Room-Temperature Fluorescence
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Solid-Phase Extraction
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Urine Analysis
dc.subject.classification
Otras Ciencias Químicas
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Room-temperature fluorescence spectroscopy of monohydroxy metabolites of polycyclic aromatic hydrocarbons on octadecyl extraction membranes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-04-05T14:31:54Z
dc.journal.volume
85
dc.journal.number
4
dc.journal.pagination
1805-1811
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Calimag Williams, Korina. University of Central Florida; Estados Unidos
dc.description.fil
Fil: Goicoechea, Hector Casimiro. University of Central Florida; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
dc.description.fil
Fil: Campiglia, Andres D.. University of Central Florida; Estados Unidos
dc.journal.title
Talanta
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.talanta.2011.07.009
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0039914011005844
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