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dc.contributor.author
Suqueli García, María Florencia
dc.contributor.author
Castellote, M.A.
dc.contributor.author
Feingold, Sergio
dc.contributor.author
Corva, Pablo Marcelo
dc.date.available
2019-04-11T14:17:28Z
dc.date.issued
2017-08
dc.identifier.citation
Suqueli García, María Florencia; Castellote, M.A.; Feingold, Sergio; Corva, Pablo Marcelo; Characterization of a deletion in the Hsp70 cluster in the bovine reference genome; Wiley Blackwell Publishing, Inc; Animal Genetics; 48; 4; 8-2017; 377-385
dc.identifier.issn
0268-9146
dc.identifier.uri
http://hdl.handle.net/11336/74024
dc.description.abstract
The 70 kilodalton heat shock proteins (Hsp70) are highly conserved molecular chaperones which have a crucial role in the stress response of the cell. In mammals, the Hsp70 proteins are encoded by a cluster of three genes: HSPA1A, HSPA1B and HSPA1L. In bovines, this cluster is located on chromosome 23 downstream of the major histocompatibility complex (BoLA). We detected inconsistencies in the location of markers on the Hsp70 genes reported in the literature that pointed to a potential deletion in the bovine reference genome UMD 3.1.1. An in silico analysis of the bovine genomic region of the Hsp70 cluster, using available information from public databases, confirmed the existence of a deletion of 11.1-kb spanning the HSPA1B gene and the intergenic region between HSPA1B and HSPA1A. Although we originally considered this an assembly error, it is most likely a particular condition of L1 Dominette 01449, the cow sequenced in the Bovine Genome Project. Moreover, we suggest a new classification of bovine Hsp70 sequences reported in NCBI and a reassignment of the location of SNPs from dbSNP that map to the deletion on BTA23. We also compared the location of selected transcription factor binding sites on the promoters of HSPA1A and HSPA1B. The results generated in the present work could be helpful to refine the reference genome of an important livestock species and also to understand the role and the regulation of the bovine Hsp70 genes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cattle
dc.subject
Genome Assembly
dc.subject
Hspa1b
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Promoter
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Snps
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Characterization of a deletion in the Hsp70 cluster in the bovine reference genome
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-03-27T14:50:40Z
dc.journal.volume
48
dc.journal.number
4
dc.journal.pagination
377-385
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Suqueli García, María Florencia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Castellote, M.A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
dc.description.fil
Fil: Feingold, Sergio. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
dc.description.fil
Fil: Corva, Pablo Marcelo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
dc.journal.title
Animal Genetics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/age.12561
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/age.12561
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