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dc.contributor.author
González Pardo, María Verónica
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dc.contributor.author
Boland, Ricardo Leopoldo
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dc.contributor.author
Russo, Ana Josefa
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dc.date.available
2019-04-11T13:31:35Z
dc.date.issued
2008-12
dc.identifier.citation
González Pardo, María Verónica; Boland, Ricardo Leopoldo; Russo, Ana Josefa; Vitamin D receptor levels and binding are reduced in aged rat intestinal subcelullar fractions; Springer; Biogerontology; 9; 2; 12-2008; 109-118
dc.identifier.issn
1389-5729
dc.identifier.uri
http://hdl.handle.net/11336/74013
dc.description.abstract
The hormonal form of vitamin D, 1α,25(OH)2-vitaminD3 [1α,25(OH)2D3], stimulates signal transduction pathways in intestinal cells. To gain insight into the relative importance of the vitamin D receptor (VDR) in the rapid hormone responses, the amounts and localization of the VDR were evaluated in young (3 months) and aged (24 months) rat intestinal cells. Immune-fluorescence and Western blot studies showed that VDR levels are diminished in aged enterocytes. Confocal microscopy assays revealed that the VDR and other immune-reactive proteins have mitochondrial, membrane, cytosol and perinuclear localization. Western blot analysis using specific antibodies detected the 60 and 50 kDa bands expected for the VDR in the cytosol and microsomes and, to a lesser extent, in the nucleus and mitochondria. Low molecular weight immune-reactive proteins were also detected in young enterocytes subcellular fractions. Since changes in hormone receptor levels appear to constitute a common manifestation of the ageing process, we also analyzed 1α,25(OH)2D3 binding properties and VDR levels in subcellular fractions from young and aged rats. In competition binding assays, employing [3H]-1α,25(OH)2D3 and 1α,25(OH)2D3, we have detected specific binding in all subcellular fractions, with maximum binding in mitochondrial and nuclear fractions. Both, VDR protein levels and 1α,25(OH)2D3 binding, were diminished with ageing. Age-related declines in VDR may have important consequences for correct receptor/effector coupling in the duodenal tissues and may explain age-related declines in the hormonal regulation of signal transduction pathways that we previously reported.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Enterocytes
dc.subject
1alfa, 25(Oh)2 D3
dc.subject
Vitamin D Receptor
dc.subject
Aging
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Vitamin D receptor levels and binding are reduced in aged rat intestinal subcelullar fractions
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-03-27T13:34:07Z
dc.identifier.eissn
1573-6768
dc.journal.volume
9
dc.journal.number
2
dc.journal.pagination
109-118
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Dordrecht
dc.description.fil
Fil: González Pardo, María Verónica. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Boland, Ricardo Leopoldo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Russo, Ana Josefa. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Biogerontology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10522-007-9118-2
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10522-007-9118-2
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