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Artículo

Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine

Vignoni, MarianaIcon ; Erra Balsells, RosaIcon ; Epe, Bernd; Cabrerizo, Franco MartínIcon
Fecha de publicación: 01/2014
Editorial: Elsevier
Revista: Journal of Photochemistry and Photobiology B: Biology
ISSN: 1011-1344
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Química Orgánica

Resumen

It is known that β-carbolines are able to produce photosensitized damage in cell-free DNA, but there is little information on their effects on cellular DNA. Therefore, we have analyzed the DNA damage produced by harmine and 9-methyl-harmine under UVA irradiation in V79 cells, together with the associated generation of micronuclei and photocytotoxicity. The results indicate that the most frequent photoproducts generated in the cellular DNA are modified purines such as 8-oxo-7,8-dihydroguanine. Only relatively few single-strand breaks were observed. CPDs were absent, although they were generated in cell-free DNA irradiated under the same conditions. The overall extent of DNA damage in the cells was considerably smaller than the one observed in cell free DNA. The generation of cellular DNA damage was associated with a significant generation of micronuclei and decreased cell proliferation. The data indicate that β-carbolines act as photosensitizers in mammalian cells. The spectrum of DNA modification, and therefore the mechanism of DNA damage generation, differs considerably from that observed with cell-free DNA.
Palabras clave: B-Carbolines , Photosensitization , Cyclobutane Pyrimidine Photodimers , Phototoxicity
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/7401
URL: http://www.sciencedirect.com/science/article/pii/S1011134414000384
DOI: http://dx.doi.org/10.1016/j.jphotobiol.2014.01.020
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(CIHIDECAR)
Articulos de CENTRO DE INVESTIGACIONES EN HIDRATOS DE CARBONO
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos(INIFTA)
Articulos de INST.DE INV.FISICOQUIMICAS TEORICAS Y APLIC.
Citación
Vignoni, Mariana; Erra Balsells, Rosa; Epe, Bernd; Cabrerizo, Franco Martín; Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine; Elsevier; Journal of Photochemistry and Photobiology B: Biology; 132; 1-2014; 66-71
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