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dc.contributor.author
Gargiulo Monachelli, Gisella Mariana
dc.contributor.author
Campos Melo, Danae
dc.contributor.author
Droppelmann, Cristian A.
dc.contributor.author
Keller, Brian A.
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Leystra Lantz, Cheryl
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de Nicola, Alejandro Federico
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Gonzalez Deniselle, Maria Claudia
dc.contributor.author
Volkening, Kathryn
dc.contributor.author
Strong, Michael J.
dc.date.available
2016-09-01T18:36:13Z
dc.date.issued
2014-02
dc.identifier.citation
Gargiulo Monachelli, Gisella Mariana; Campos Melo, Danae; Droppelmann, Cristian A.; Keller, Brian A.; Leystra Lantz, Cheryl; et al.; Expression and cellular localization of the classical progesterone receptor in healthy and amyotrophic lateral sclerosis affected spinal cord; Wiley; European Journal Of Neurology; 21; 2; 2-2014; 273-280
dc.identifier.issn
1351-5101
dc.identifier.uri
http://hdl.handle.net/11336/7377
dc.description.abstract
Background and purpose Previous studies have suggested that elevated progesterone levels are associated with a slower disease course in amyotrophic lateral sclerosis (ALS). Given that the effects of progesterone are mediated in part by the classical progesterone receptor (PR), the expression and cellular localization of the A and B isoforms (PR-A and PR-B, respectively) of the PR in control (neuropathologically normal) and ALS-affected spinal cord (SC) were examined. Methods Semi-quantitative RT-PCR, immunohistochemistry and immunofluorescence analyses of the cervical and lumbar SC of post-mortem ALS patients (n = 19) and control subjects (n = 10) were performed. Primers and antibodies used allowed the detection of both PR-A and PR-B isoforms together (PR-A+B) or PR-B isoform alone. Results Lumbar PR-A+B and cervical PR-B mRNA expression were significantly higher in ALS than controls. In both ALS and controls, PR-A+B immunoreactivity (IR) was occasionally detected in motor neurons. In contrast, PR-A+B IR was prominent in axonal processes and vessels. This was more evident in nerve roots and large arteries in ALS compared with controls. Colocalization of PR-A+B with markers of neurons, axonal processes and vascular endothelium was also observed. Conclusions Evidence that both PR-A and PR-B isoforms are expressed in the human SC is provided, with some regional variation in isoform expression between ALS and controls. The IR was more prominent in nerve roots and large arteries in ALS, suggesting a potential role in the degenerative process.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Amyotrophic Lateral Sclerosis
dc.subject
Progesterone Receptor
dc.subject.classification
Neurociencias
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Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Expression and cellular localization of the classical progesterone receptor in healthy and amyotrophic lateral sclerosis affected spinal cord
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-06-15T19:10:55Z
dc.journal.volume
21
dc.journal.number
2
dc.journal.pagination
273-280
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Gargiulo Monachelli, Gisella Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Western University; Reino Unido
dc.description.fil
Fil: Campos Melo, Danae. Western University; Reino Unido
dc.description.fil
Fil: Droppelmann, Cristian A.. Western University; Reino Unido
dc.description.fil
Fil: Keller, Brian A.. Western University; Reino Unido
dc.description.fil
Fil: Leystra Lantz, Cheryl. Western University; Reino Unido
dc.description.fil
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Gonzalez Deniselle, Maria Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Volkening, Kathryn. Western University; Reino Unido
dc.description.fil
Fil: Strong, Michael J.. Western University; Reino Unido
dc.journal.title
European Journal Of Neurology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ene.12291/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/ene.12291


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