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dc.contributor.author
Rey, Mariana
dc.contributor.author
Kruse, Maria Sol
dc.contributor.author
Alvarez, Lautaro Damian
dc.contributor.author
Ghini, Alberto Antonio
dc.contributor.author
Veleiro, Adriana Silvia
dc.contributor.author
Burton, Gerardo
dc.contributor.author
Coirini, Hector
dc.date.available
2016-08-30T18:04:25Z
dc.date.issued
2013-08
dc.identifier.citation
Rey, Mariana; Kruse, Maria Sol; Alvarez, Lautaro Damian; Ghini, Alberto Antonio; Veleiro, Adriana Silvia; et al.; Neuroprotective action of synthetic steroids with oxygen bridge. Activity on GABAA receptor; Elsevier; Experimental Neurology; 249; 8-2013; 49-58
dc.identifier.issn
0014-4886
dc.identifier.uri
http://hdl.handle.net/11336/7354
dc.description.abstract
Allopregnanolone (A) and pregnanolone (P) are able tomodify neural activities acting through the GABAA receptor complex. This capacity makes them useful as anticonvulsant, anxiolytic, or  anti-stress compounds. In this study, the performance of seven synthetic steroids (SS) analogous of A or P containing an intramolecular oxygen bridge was evaluated using different assays. Competition assays showed that compounds 1, 5, 6 and 7 affected the binding of specific ligands for the GABAA receptor in a way similar to that of A and P, whereas compounds 3 and 4 stimulated [3H]-flunitrazepamand reduced [35S]-TBPS binding. The enzyme3β-hydroxysteroid dehydrogenase (3β-HSD) produces the precursor for A and P, and its activity is regulated by steroids. The action of several SS on 3β-HSD activity was tested in different tissues. All SS analyzed inhibit its activity, but compound 5 was the least effective. Finally, the neuroprotective role of two SSwas evaluated in cerebral cortex and hippocampus cultures subjected to hypoxia. Glial fibrillary acidic protein (GFAP) increasewas prevented by A, P, and compounds 3 and 5. Only A, P and compound 5 prevented neurofilament (NF160/200) decrease in hippocampus cultures,<br />whereas A and compound 5 partially prevented NF200 and NF160 decreases respectively in cerebral cortex cultures. A preventedmicrotubule associated protein (MAP 2b) decrease in cerebral cortex cultures,while in hippocampus cultures only compounds 3 and 5 had effect. All steroids prevented MAP 2c decrease in both brain regions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Neurosteroids
dc.subject
3b-Hsd
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Hypoxia
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Hyppocampus
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Hypothalamus
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Cerebellum
dc.subject.classification
Neurociencias
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Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Neuroprotective action of synthetic steroids with oxygen bridge. Activity on GABAA receptor
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-05-10T13:47:18Z
dc.journal.volume
249
dc.journal.pagination
49-58
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Rey, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Kruse, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Alvarez, Lautaro Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos Aplicados A la Química Orgánica (i); Argentina
dc.description.fil
Fil: Ghini, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos Aplicados A la Química Orgánica (i); Argentina
dc.description.fil
Fil: Veleiro, Adriana Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos Aplicados A la Química Orgánica (i); Argentina
dc.description.fil
Fil: Burton, Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos Aplicados A la Química Orgánica (i); Argentina
dc.description.fil
Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.journal.title
Experimental Neurology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014488613002379
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.expneurol.2013.07.020


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