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dc.contributor.author
Varni, Vanina Delia
dc.contributor.author
Ruybal, Paula
dc.contributor.author
Lauthier, Juan José
dc.contributor.author
Tomasini, Nicolás
dc.contributor.author
Brihuega, Bibiana
dc.contributor.author
Koval, Ariel
dc.contributor.author
Caimi, Karina Cynthia
dc.date.available
2016-08-29T19:03:45Z
dc.date.issued
2014-03
dc.identifier.citation
Varni, Vanina Delia; Ruybal, Paula; Lauthier, Juan José; Tomasini, Nicolás; Brihuega, Bibiana; et al.; Reassessment of MLST schemes for Leptospira spp. typing worldwide; Elsevier; Infection, Genetics And Evolution; 22; 3-2014; 216-222
dc.identifier.issn
1567-1348
dc.identifier.uri
http://hdl.handle.net/11336/7338
dc.description.abstract
Leptospirosis is a neglected zoonosis of global importance. Several multilocus sequence typing (MLST) methods have been developed for Leptospira spp., the causative agent of leptospirosis. In this study we reassessed the most commonly used MLST schemes in a set of worldwide isolates, in order to select the loci that achieve the maximum power of discrimination for typing Leptospira spp. Global eBURST algorithm was used to detect clonal complexes among STs and phylogenetic relationships among concatenated and individual sequences were inferred through maximum likelihood (ML) analysis. The evaluation of 12 loci combined to type a subset of strains rendered 57 different STs. Seven of these loci were selected into a final scheme upon studying the number of alleles and polymorphisms, the typing efficiency, the discriminatory power and the ratio dN/dS per nucleotide site for each locus. This new 7-locus scheme was applied to a wider collection of worldwide strains. The ML tree constructed from concatenated sequences of the 7 loci identified 6 major clusters corresponding to 6 Leptospira species. Global eBURST established 8 CCs, which showed that genotypes were clearly related by geographic origin and host. ST52 and ST47, represented mostly by Argentinian isolates, grouped the higher number of isolates. These isolates were serotyped as serogroups Pomona and Icterohaemorrhagiae, showing a unidirectional correlation in which the isolates with the same ST belong to the same serogroup. In summary, this scheme combines the best loci from the most widely used MLST schemes for Leptospira spp. and supports worldwide strains classification. The Argentinian isolates exhibited congruence between allelic profile and serogroup, providing an alternative to serological methods.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Mlst
dc.subject
Leptospira
dc.subject
Typing
dc.subject
Reassessment
dc.subject.classification
Enfermedades Infecciosas
dc.subject.classification
Ciencias de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.subject.classification
Epidemiología
dc.subject.classification
Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
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Biología Celular, Microbiología
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Reassessment of MLST schemes for Leptospira spp. typing worldwide
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-08-04T18:20:08Z
dc.journal.volume
22
dc.journal.pagination
216-222
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Varni, Vanina Delia. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
dc.description.fil
Fil: Lauthier, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
dc.description.fil
Fil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
dc.description.fil
Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Koval, Ariel. Biogenesis Bago S.a; Argentina
dc.description.fil
Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.journal.title
Infection, Genetics And Evolution
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567134813002980
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.meegid.2013.08.002


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