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dc.contributor.author
Sala, Juan Manuel
dc.contributor.author
Trotta, Myrian Vanesa
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Mansilla, Florencia Celeste
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Pérez Filgueira, Daniel Mariano
dc.contributor.author
Caspe, Sergio Gastón
dc.contributor.author
Capozzo, Alejandra Victoria
dc.date.available
2019-04-03T15:25:02Z
dc.date.issued
2018-06
dc.identifier.citation
Sala, Juan Manuel; Trotta, Myrian Vanesa; Mansilla, Florencia Celeste; Pérez Filgueira, Daniel Mariano; Caspe, Sergio Gastón; et al.; Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis); Garuda Scientific Publications; Journal Of Applied Animal Research; 46; 1; 6-2018; 451-458
dc.identifier.issn
0971-2119
dc.identifier.uri
http://hdl.handle.net/11336/73035
dc.description.abstract
Buffaloes are compulsory vaccinated against foot-and-mouth disease virus (FMDV) in many countries as part of the official control programmes. Serological testing aimed to indirectly assess herd immunity is currently performed using the same Enzyme-linked immunosorbent assays (ELISAs) applied for bovine sera, assuming an agreement between the ELISA’s diagnostic results and those obtained using the virus neutralization test (VNT). Here we evaluated the accuracy of different ELISA tests to assess vaccine-induced antibodies against FMDV in buffalo’s sera classified according to their VNT titres. Currently used liquid-phase blocking ELISA yielded very low specificity, producing high titres for many samples with low VNT titres. To increase specificity, we developed an indirect ELISA using purified 140S viral particles and an avidity single-dilution ELISA, which includes a urea washing step after the incubation of the diluted serum sample, to detach weak binders. Combining these two highthroughput single-dilution tests, an excellent concordance with VNT was achieved. This is the first study analysing the diagnostic agreement of traditional and novel serological tests with VNT for the indirect assessment of antibodies against FMDV capsid proteins in buffalo serum samples.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Garuda Scientific Publications
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Buffaloes
dc.subject
Foot-And-Mouth Disease Virus
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Serological Tests
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Vaccine
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Otras Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-03-26T14:14:43Z
dc.journal.volume
46
dc.journal.number
1
dc.journal.pagination
451-458
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Corrientes. Estación Experimental Agropecuaria Mercedes; Argentina
dc.description.fil
Fil: Trotta, Myrian Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Mansilla, Florencia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Pérez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Caspe, Sergio Gastón. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Corrientes. Estación Experimental Agropecuaria Mercedes; Argentina
dc.description.fil
Fil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.journal.title
Journal Of Applied Animal Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09712119.2017.1335641
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1080/09712119.2017.1335641
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