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dc.contributor.author
Caballero, Beatriz
dc.contributor.author
Olguin, Nair Temis
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dc.contributor.author
Campos, Francisco
dc.contributor.author
Farina, Marcelo
dc.contributor.author
Ballester, Ferran
dc.contributor.author
Lopez Espinosa, Maria Jose
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dc.contributor.author
Llop, Sabrina
dc.contributor.author
Rodríguez Farré, Eduard
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dc.contributor.author
Suñol, Cristina
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dc.date.available
2019-04-01T14:10:33Z
dc.date.issued
2017-03
dc.identifier.citation
Caballero, Beatriz; Olguin, Nair Temis; Campos, Francisco; Farina, Marcelo; Ballester, Ferran; et al.; Methylmercury-induced developmental toxicity is associated with oxidative stress and cofilin phosphorylation. Cellular and human studies; Elsevier Science; Neurotoxicology; 59; 3-2017; 197-209
dc.identifier.issn
0161-813X
dc.identifier.uri
http://hdl.handle.net/11336/72899
dc.description.abstract
Environmental exposure to methylmercury (MeHg) during development is of concern because it is easily incorporated in children's body both pre- and post-natal, it acts at several levels of neural pathways (mitochondria, cytoskeleton, neurotransmission) and it causes behavioral impairment in child. We evaluated the effects of prolonged exposure to 10–600 nM MeHg on primary cultures of mouse cortical (CCN) and of cerebellar granule cells (CGC) during their differentiation period. In addition, it was studied if prenatal MeHg exposure correlated with altered antioxidant defenses and cofilin phosphorylation in human placentas (n = 12) from the INMA cohort (Spain). Exposure to MeHg for 9 days in vitro (DIV) resulted in protein carbonylation and in cell death at concentrations ≥200 nM and ≥300 nM, respectively. Exposure of CCN and CGC to non-cytotoxic MeHg concentrations for 5 DIV induced an early concentration-dependent decrease in cofilin phosphorylation. Furthermore, in both cell types actin was translocated from the cytosol to the mitochondria whereas cofilin translocation was found only in CGC. Translocation of cofilin and actin to mitochondria in CGC occurred from 30 nM MeHg onwards. We also found an increased expression of cortactin and LIMK1 mRNA in CGC but not in CCN. All these effects were prevented by the antioxidant probucol. Cofilin phosphorylation was significantly decreased and a trend for decreased activity of glutathione reductase and glutathione peroxidase was found in the fetal side of human placental samples from the highest (20–40 μg/L) MeHg-exposed group when compared with the low (<7 μg/L) MeHg-exposed group. In summary, cofilin dephosphorylation and oxidative stress are hallmarks of MeHg exposure in both experimental and human systems.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Actin
dc.subject
Cofilin
dc.subject
Cultured Neurons
dc.subject
Human Placenta
dc.subject
Methylmercury
dc.subject
Mitochondria
dc.subject
Oxidative Stress
dc.subject.classification
Salud Ocupacional
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dc.subject.classification
Ciencias de la Salud
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Methylmercury-induced developmental toxicity is associated with oxidative stress and cofilin phosphorylation. Cellular and human studies
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-03-29T12:06:12Z
dc.journal.volume
59
dc.journal.pagination
197-209
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Caballero, Beatriz. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Olguin, Nair Temis. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Campos, Francisco. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Farina, Marcelo. Universidade Federal de Santa Catarina; Brasil. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Ballester, Ferran. Universidad de Valencia; España. Universitat Jaume I; España
dc.description.fil
Fil: Lopez Espinosa, Maria Jose. Universidad de Valencia; España. Universitat Jaume I; España
dc.description.fil
Fil: Llop, Sabrina. Universidad de Valencia; España. Universitat Jaume I; España
dc.description.fil
Fil: Rodríguez Farré, Eduard. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Suñol, Cristina. Consejo Superior de Investigaciones Científicas; España
dc.journal.title
Neurotoxicology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.neuro.2016.05.018
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0161813X16300961
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