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dc.contributor.author
Dal Zotto, A.
dc.contributor.author
Cardozo, A.
dc.contributor.author
Cabrera Mederos, Dariel
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Nome, Claudia
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Giolitti, Fabián José
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Cobelo, Claudia Mónica
dc.date.available
2019-03-28T18:18:34Z
dc.date.issued
2017-11
dc.identifier.citation
Dal Zotto, A.; Cardozo, A.; Cabrera Mederos, Dariel; Nome, Claudia; Giolitti, Fabián José; et al.; First report of raspberry bushy dwarf virus infecting raspberry in Argentina; Edizioni Ets; Journal Of Plant Pathology; 99; 2; 11-2017
dc.identifier.issn
1125-4653
dc.identifier.uri
http://hdl.handle.net/11336/72753
dc.description.abstract
In 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in commercial fields in parallel 42° in the Patagonia region of Argentina. Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA (Ellis et al., 2005; Martin et al., 2013). A survey was conducted to determine if RBDV was also present in Argentinian raspberry fields. Transmission electron microscope observations of leaf dip preparations revealed the presence of spherical particles of 30 nm, resembling those of virus members of the genus Ideaovirus. RBDV was detected in 88 of the 130 plants (68%) tested using double-antibody sandwich ELISA with a specific polyclonal antiserum (Bioreba AG, Switzerland). RNA was extracted with SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA) and tested by RT-PCR using a pair of specific primers to the capsid protein gene (Wang et al., 2008). Expected fragments of 825 bp were obtained and custom-sequenced in both directions (Macrogen, Korea). Nucleotide BLAST analysis of the complete sequence obtained (GenBank accession No. KY308191) showed 97% identity at the nucleotide level with virus isolates from Slovenia, Belarus, Switzerland and Japan (EU796088, FR687356, FR687358, AB948216, respectively). To our knowledge, this is the first report of RBDV infecting raspberry in Argentina. Further research is needed to determine the distribution of this virus in the country and its effects on raspberry plant production.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Edizioni Ets
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Raspberry Bushy Dwarf Virus
dc.subject
Argentina
dc.subject.classification
Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
First report of raspberry bushy dwarf virus infecting raspberry in Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-03-18T19:27:47Z
dc.journal.volume
99
dc.journal.number
2
dc.journal.pais
Italia
dc.journal.ciudad
Bari
dc.description.fil
Fil: Dal Zotto, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.description.fil
Fil: Cardozo, A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Agencia de Extensión Rural El Bolsón; Argentina
dc.description.fil
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Nome, Claudia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.description.fil
Fil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.description.fil
Fil: Cobelo, Claudia Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Agencia de Extensión Rural El Bolsón; Argentina
dc.journal.title
Journal Of Plant Pathology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sipav.org/main/jpp/index.php/jpp/article/view/3896
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4454/jpp.v99i2.3896
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