Artículo
Tubulin is retained throughout the human hematopoietic/erythroid cell differentiation process and plays a structural role in sedimentable fraction of mature erythrocytes
Nigra, Ayelén Denise
; Santander, Verónica Silvina
; Dircio Maldonado, Roberto; Amaiden, Marina Rafaela
; Monesterolo, Noelia Edith
; Flores Guzmán, Patricia; Muhlberger, Tamara
; Rivelli Antonelli, Juan Franco
; Campetelli, Alexis Nazareno
; Mayani, Héctor; Casale, Cesar Horacio
Fecha de publicación:
10/2017
Editorial:
Pergamon-Elsevier Science Ltd
Revista:
International Journal of Biochemistry and Cellular Biology
ISSN:
1357-2725
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We investigated the properties of tubulin present in the sedimentable fraction (“Sed-tub”) of human erythrocytes, and tracked the location and organization of tubulin in various types of cells during the process of hematopoietic/erythroid differentiation. Sed-tub was sensitive to taxol/nocodazole (drugs that modify microtubule assembly/disassembly), but was organized as part of a protein network rather than in typical microtubule form. This network had a non-uniform “connected-ring” structure, with tubulin localized in the connection areas and associated with other proteins. When tubulin was eliminated from Sed-tub fraction, this connected-ring structure disappeared. Spectrin, a major protein component in Sed-tub fraction, formed a complex with tubulin. During hematopoietic differentiation, tubulin shifts from typical microtubule structure (in pro-erythroblasts) to a disorganized structure (in later stages), and is retained in reticulocytes following enucleation. Thus, tubulin is not completely lost when erythrocytes mature; it continues to play a structural role in the Sed-tub fraction.
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Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Nigra, Ayelén Denise; Santander, Verónica Silvina; Dircio Maldonado, Roberto; Amaiden, Marina Rafaela; Monesterolo, Noelia Edith; et al.; Tubulin is retained throughout the human hematopoietic/erythroid cell differentiation process and plays a structural role in sedimentable fraction of mature erythrocytes; Pergamon-Elsevier Science Ltd; International Journal of Biochemistry and Cellular Biology; 91; 10-2017; 29-36
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