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Artículo

Modulating surface density of proteins via caged surfaces and controlled light exposure

Álvarez, Marta; Alonso, José María; Filevich, OscarIcon ; Bhagawati, Maniraj; Etchenique, Roberto ArgentinoIcon ; Piehler, Jacob; del Campo, Aránzazu
Fecha de publicación: 03/2011
Editorial: American Chemical Society
Revista: Langmuir
ISSN: 0743-7463
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Químicas

Resumen

We demonstrate the possibility of tuning the degree of functionalization of a surface using photoactivatable chemistries and controlled light exposure. A photosensitive organosilane with a protected amine terminal group and a tetraethyleneglycol spacer was synthesized. A o-nitrobenzyl cage was used as the photoremovable group to cage the amine functionality. Surfaces with phototunable amine densities were generated by controlled irradiation of silica substrates modified with the photosensitive anchor. Protein layers with different densities could be obtained by successive coupling and assembly steps. Protein surface concentrations were quantified by reflectance interference. Our results demonstrate that the protein density correlates with the photogenerated ligand density. The density control was proved over four coupling steps (biotin, SAv, BTtris-NTA, MBP, or GFP), indicating that the interactions between underlying layer and soluble targets are highly specific and the immobilized targets at the four levels maintain their full functionality. Protein micropatterns with a gradient of protein density were also obtained. © 2011 American Chemical Society.
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/71949
URL: https://pubs.acs.org/doi/pdf/10.1021/la104511x
DOI: https://dx.doi.org/10.1021/la104511x
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Articulos(INQUIMAE)
Articulos de INST.D/QUIM FIS D/L MATERIALES MEDIOAMB Y ENERGIA
Citación
Álvarez, Marta; Alonso, José María; Filevich, Oscar; Bhagawati, Maniraj; Etchenique, Roberto Argentino; et al.; Modulating surface density of proteins via caged surfaces and controlled light exposure; American Chemical Society; Langmuir; 27; 6; 3-2011; 2789-2795
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