Artículo

Automated image analysis for monitoring oxidative burst in macrophages

Molinari, Beatriz LuciaIcon ; Tasat, Deborah Ruth; Fernández, María LauraIcon ; Duran, Hebe AliciaIcon ; Curiale, J.; Stoliar, A.; Cabrini, Rómulo L.
Fecha de publicación: 12/2000
Editorial: International Academy of Cytology and the Italian Society of Urologic Pathology
Revista: Analytical and Quantitative Cytology and Histology
ISSN: 0884-6812
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

OBJECTIVE: To evaluate oxidative bursts induced by phorbol myristate acetate in phagocytes at the single-cell level by automated image analysis. STUDY DESIGN: The generation of reactive oxygen species was quantitatively expressed by means of histograms displaying the percentage of cells corresponding to each of the total optical densities measured. RESULTS: Macrophage subpopulations were quantitatively defined. This method allows detailed analysis of the amount of formazan per cell and the sites of deposition of blue precipitate in each cell. CONCLUSION: Image analysis is a reliable quantitative, single-cell assay for studying various cellular characteristics associated with macrophage functions.
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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URI: http://hdl.handle.net/11336/71938
URL: http://www.aqch.com/toc/auto_abstract.php?id=15163
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Molinari, Beatriz Lucia; Tasat, Deborah Ruth; Fernández, María Laura; Duran, Hebe Alicia; Curiale, J.; et al.; Automated image analysis for monitoring oxidative burst in macrophages; International Academy of Cytology and the Italian Society of Urologic Pathology; Analytical and Quantitative Cytology and Histology; 22; 5; 12-2000; 423-427
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