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dc.contributor.author
Suarez, Carlos E.
dc.contributor.author
Jacobsen, Monica Ofelia
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dc.contributor.author
Hines, Stephen A.
dc.contributor.author
Palmer, Guy H.
dc.contributor.author
Brown, Wendy C.
dc.contributor.author
McElwain, Terry F.
dc.date.available
2019-03-14T20:55:41Z
dc.date.issued
2000-12
dc.identifier.citation
Suarez, Carlos E.; Jacobsen, Monica Ofelia; Hines, Stephen A.; Palmer, Guy H.; Brown, Wendy C.; et al.; Characterization of allelic variation in the babesia bovis merozoite surface antigen 1 (MSA-1) locus and identification of a cross-reactive inhibition-sensitive msa-1 epitope; American Society for Microbiology; Infection and Immunity; 68; 12; 12-2000; 6865-6870
dc.identifier.issn
0019-9567
dc.identifier.uri
http://hdl.handle.net/11336/71717
dc.description.abstract
The Babesia bovis merozoite surface antigen 1 (MSA-1), a member of the variable merozoite surface antigen (VMSA) family, is an immunodominant glycoprotein which elicits antibodies that inhibit erythrocyte invasion. While antigenic polymorphism is a general feature of vmsa genes, the molecular basis and extent of msa-1 sequence polymorphism have not been well characterized. In this study we defined the msa-1 locus in the biologically cloned Mexico Mo7 strain of B. bovis and identified the sequence differences between MSA-1 antigenically dissimilar strains. We then determined whether sequences conserved between distinct msa-1 alleles would induce cross-reactive CD4+ T lymphocytes or inhibitory antibodies. The msa-1 locus in Mo7 contains a single msa-1 gene flanked by transcribed genes with no sequence homology to members of the VMSA gene family. Argentina B. bovis strains R1A and S2P have msa-1 genes with amino acid sequences that are 98.8% identical to each other, and antibodies against S2P MSA-1 cross-react with native R1A MSA-1. In contrast, identity between the Argentina and Mexico Mo7 msa-1 alleles is only 52%, with no continuous stretch of identity longer than 16 amino acids. Despite limited sequence conservation, antibodies against R1A MSA-1 were able to inhibit invasion of erythrocytes by Mo7 merozoites. The results indicate that inhibition-sensitive epitopes are conserved despite significant sequence divergence between Mexico and Argentina strain alleles and support a conserved functional role for polymorphic MSA-1 in erythrocyte invasion.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Characterization of allelic variation in the babesia bovis merozoite surface antigen 1 (MSA-1) locus and identification of a cross-reactive inhibition-sensitive msa-1 epitope
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-03-14T14:09:38Z
dc.journal.volume
68
dc.journal.number
12
dc.journal.pagination
6865-6870
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Suarez, Carlos E.. Washington State University; Estados Unidos
dc.description.fil
Fil: Jacobsen, Monica Ofelia. Washington State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Hines, Stephen A.. Washington State University; Estados Unidos
dc.description.fil
Fil: Palmer, Guy H.. Washington State University; Estados Unidos
dc.description.fil
Fil: Brown, Wendy C.. Washington State University; Estados Unidos
dc.description.fil
Fil: McElwain, Terry F.. Washington State University; Estados Unidos
dc.journal.title
Infection and Immunity
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/IAI.68.12.6865-6870.2000
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/10.1128/IAI.68.12.6865-6870.2000
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC97791/
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