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Artículo

A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections

Osterman, Andreas; Vizoso Pinto, María GuadalupeIcon ; Jung, Jette; Jaeger, Gundula; Eberle, Josef; Nitschko, Hans; Baiker, Armin
Fecha de publicación: 04/2013
Editorial: Elsevier
Revista: Journal Of Virological Methods
ISSN: 0166-0934
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Virología; Tecnologías que involucran la identificación de ADN, proteínas y enzimas, y cómo influyen en el conjunto de enfermedades y mantenimiento del bienestar; Pediatría

Resumen

Hepatitis E Virus (HEV) causes epidemic infections in regions of poor hygiene in the developing world. Over the last years, however, increasing numbers of autochthonous infections in industrialized countries have been described, leading to new interest in this pathogen. Currently available serological test formats to detect IgG and IgM antibodies are mainly based on bacterially expressed ORF2 and ORF3 antigens and often give ambiguous results. The objective of this study was the development of a different assay format for HEV diagnosis—a HEV immunofluorescence test (HEV-IFT) based on mammalian cells transiently expressing recombinant HEV ORF2 protein with a simple production and staining protocol and the investigation of its performance and methodical feasibility under diagnostic laboratory conditions. 31 sera of patients at different phases of HEV infection and 40 control sera from a non-endemic region were analyzed for anti-HEV IgG, IgM, and IgA antibodies. The HEV-IFT detected successfully anti-HEV IgG and IgA, but not anti-HEV IgM antibodies. In the study group the HEV-IFT was able to confirm HEV infections and to support diagnosis when ambiguous results were obtained by commercial assays. Signal localization and staining patterns helped to gather additional information about reactive antibodies present in patient sera. In conclusion the developed IFT for the detection of anti-HEV IgG and IgA antibodies can be used for diagnosis and for the serological confirmation of HEV infections.
Palabras clave: Hepatitis e Virus , Immunofluorescense , Serological Test , Orf2
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/7170
DOI: http://dx.doi.org/10.1016/j.jviromet.2013.03.022
URL: http://www.sciencedirect.com/science/article/pii/S0166093413000980
Colecciones
Articulos(CCT - NOA SUR)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Citación
Osterman, Andreas; Vizoso Pinto, María Guadalupe; Jung, Jette; Jaeger, Gundula; Eberle, Josef; et al.; A novel indirect immunofluorescence test for the detection of IgG and IgA antibodies for diagnosis of Hepatitis E Virus infections; Elsevier; Journal Of Virological Methods; 191; 1; 4-2013; 48-54
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