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dc.contributor.author
Sgariglia, Melina Araceli
dc.contributor.author
Soberon, Jose Rodolfo
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Poveda Cabanes, Ana
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Sampietro, Diego Alejandro
dc.contributor.author
Vattuone, Marta Amelia
dc.date.available
2016-08-16T15:31:04Z
dc.date.issued
2013-05
dc.identifier.citation
Sgariglia, Melina Araceli; Soberon, Jose Rodolfo; Poveda Cabanes, Ana; Sampietro, Diego Alejandro; Vattuone, Marta Amelia; Anti-inflammatory properties of phenolic lactones isolated from Caesalpinia paraguariensis stem bark; Elsevier Ireland; Journal Of Ethnopharmacology; 147; 1; 5-2013; 63-73
dc.identifier.issn
0378-8741
dc.identifier.uri
http://hdl.handle.net/11336/7166
dc.description.abstract
Ethnopharmacological relevance Caesalpinia paraguariensis (D. Parodi) Burkart stem bark infusion (CPBI) is traditionally used in Argentina because their “vulnerary” properties. Aim of the study CPBI was studied throughout bio-guided purification procedures conducted by in vitro biological assays in order to isolate the main bioactive compounds. Material and methods Anti-inflammatory activity was assessed by enzyme inhibition assays of Hyaluronidase (Hyal) and inducible Nitric Oxide Synthase (iNOS). The antioxidant properties were evaluated by DPPH free radical scavenging assay, lipid peroxidation inhibition assay on erythrocyte membranes, and a cell-based assay that included the fluorescent probe (DCFH-DA) for indicating reactive oxygen species (ROS) generation. Bioactive compounds were purified by chromatographic methods and their structures elucidated using spectroscopic methods (ESI-MS and 1D/2D-1H/13C-NMR). Results Four main bioactive compounds were isolated from CPBI: ellagic acid (1), 3-O-methylellagic acid (2), 3,3'-di-O-methylellagic acid (3) and 3,3'-di-O-methylellagic-4-β-D-xylopyranoside (4). These were bioactive at concentrations in which are present in CPBI, being compounds 2 and 3 the best enzyme inhibitors of Hyal and iNOS, reaching the 90% inhibitory concentration (IC90) values ranging from 2.8 to 16.4 μM, that are better than that of the positive controls, aspirin (IC90: no reached) and aminoguanidine (IC90: 20.2 μM) respectively. Compounds 2 and 3 were also better scavengers for lipoperoxides than butylated hydroxytoluene (BHT), reaching the 90% effective concentration (EC90) at 1.2–4.5 μg/ml, and for DPPH radical (2.5–7.3 μg/ml); moreover compounds were able to exert its scavenging action on intracellular ROS. Structural features relevant to the biological activities are discussed. Conclusions This work provides scientific validity to the popular usage of CPBI.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Caesalpinia Paraguariensis
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Corteza
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Antiinflamatorios
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Antioxidantes
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Farmacología y Farmacia
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Anti-inflammatory properties of phenolic lactones isolated from Caesalpinia paraguariensis stem bark
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-08-11T19:37:30Z
dc.journal.volume
147
dc.journal.number
1
dc.journal.pagination
63-73
dc.journal.pais
Irlanda
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Sgariglia, Melina Araceli. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina
dc.description.fil
Fil: Soberon, Jose Rodolfo. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Estudios Vegetales. Catedra de Fitoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina
dc.description.fil
Fil: Poveda Cabanes, Ana. Universidad Autónoma de Madrid; España
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Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Estudios Vegetales. Catedra de Fitoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina
dc.description.fil
Fil: Vattuone, Marta Amelia. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Estudios Vegetales. Catedra de Fitoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina
dc.journal.title
Journal Of Ethnopharmacology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378874112008641
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jep.2012.12.030
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jep.2012.12.030