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dc.contributor.author
Mazzucotelli, Cintia Anabela
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dc.contributor.author
Agüero, Maria Victoria
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dc.contributor.author
Moreira, Maria del Rosario
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dc.contributor.author
Ansorena, Maria Roberta
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dc.date.available
2019-03-13T20:35:01Z
dc.date.issued
2016-05
dc.identifier.citation
Mazzucotelli, Cintia Anabela; Agüero, Maria Victoria; Moreira, Maria del Rosario; Ansorena, Maria Roberta; Optimization of medium components and physicochemical parameters to simultaneously enhance microbial growth and production of lypolitic enzymes by Stenotrophomonas sp.; Portland Press; Biotechnology and Applied Biochemistry; 63; 3; 5-2016; 407-418
dc.identifier.issn
0885-4513
dc.identifier.uri
http://hdl.handle.net/11336/71554
dc.description.abstract
The optimization of lipase and esterase production (LP and EP) and bacterial growth (BG) of a Stenotrophomonas sp. strain was developed. For this purpose, the effect of five different medium components and three physicochemical parameters were evaluated using a Plackett–Burman statistical design. Among eight variables, stirring speed, pH, and peptone concentration were found to be the most effective factors on the three responses under evaluation. An optimization study applying Box–Behnken response surface methodology was used to study the interactive effects of the three selected variables on LP/EP and microorganism growth. Predicted models were found to be significant with high regression coefficients (90%–99%). By using the desirability function approach, the optimum condition applying simultaneous optimization of the three responses under study resulted to be: stirring speed of 100 rpm, pH of 7.5, and a peptone concentration of 10 g/L, with a desirability value of 0.977. Under these optimal conditions, it is possible to achieve in the optimized medium a 15-fold increase in esterase productivity, a 117-fold increase in lipase production, and a 9-log CFU/mL increase in BG, compared with the basal medium without agitation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Portland Press
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Lipase
dc.subject
Medium Optimization
dc.subject
Plackettburman Design
dc.subject
Response Surface Methodology
dc.subject
Stenotrophomonas Sp
dc.title
Optimization of medium components and physicochemical parameters to simultaneously enhance microbial growth and production of lypolitic enzymes by Stenotrophomonas sp.
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-03-06T13:53:25Z
dc.journal.volume
63
dc.journal.number
3
dc.journal.pagination
407-418
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Mazzucotelli, Cintia Anabela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina
dc.description.fil
Fil: Agüero, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ingeniería; Argentina
dc.description.fil
Fil: Moreira, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina
dc.description.fil
Fil: Ansorena, Maria Roberta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina
dc.journal.title
Biotechnology and Applied Biochemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1002/bab.1378
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/bab.1378
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