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dc.contributor.author
Bermejo, Emilse  
dc.contributor.author
Alberto, Maria Fabiana  
dc.contributor.author
Meschengieser, Susana S.  
dc.contributor.author
Lazzari, María Ángela  
dc.date.available
2019-03-11T15:00:06Z  
dc.date.issued
2004-04  
dc.identifier.citation
Bermejo, Emilse; Alberto, Maria Fabiana; Meschengieser, Susana S.; Lazzari, María Ángela; Assessment of platelet activation in myeloproliferative disorders with complementary techniques; Lippincott Williams; Blood Coagulation & Fibrinolysis : An International Journal In Haemostasis And Thrombosis.; 15; 3; 4-2004; 235-240  
dc.identifier.isbn
0957-5235  
dc.identifier.issn
0957-5235  
dc.identifier.uri
http://hdl.handle.net/11336/71329  
dc.description.abstract
Bleeding and thrombosis in myeloproliferative disorders (MPD) are common events, sometimes both are present in the same patient during the course of the disease. Platelet activation in patients with MPD is often suggested. The present study analyses the presence of circulating activated platelets, using simultaneously flow cytometry and aggregometric studies in MPD. We studied 28 patients: 13 with polycythaemia vera, seven with essential thrombocythaemia, and eight chronic myeloid leukaemia. We performed functional tests, aggregation and adenosine triphosphate (ATP) release and flow cytometric assays (mepacrine staining and platelet activation markers CD62, CD63 and fibrinogen binding (B-FG)). Twenty-one MPD samples (75%) had reduced aggregation and ATP release. Acquired δ-SPD was detected in 11 of 28 MPD patients (39%), and we found no association between reduced mepacrine labelling and abnormal ATP release. High levels of activation markers were obtained: CD62 in 19 of 28 patients (68%), CD63 in 13 of 28 patients (46%) and B-FG in 19 of 28 patients (68%). The most prevalent abnormality was a reduced aggregation and ATP release. The lack of association between ATP release and mepacrine labelling suggests that other mechanisms, besides the deficit of intraplatelet ATP/adenosine diphosphate, might occur. High levels of activation markers were also observed. We conclude that both tests are complementary and necessary to understand the functional status of platelets in MPD.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Lippincott Williams  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Activated Platelets  
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Aggregation  
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Flow Cytometry  
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Myeloproliferative Disorders  
dc.subject
Storage Pool Disease  
dc.subject.classification
Inmunología  
dc.subject.classification
Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
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Hematología  
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Medicina Clínica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Assessment of platelet activation in myeloproliferative disorders with complementary techniques  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-11-22T14:57:21Z  
dc.identifier.eissn
1473-5733  
dc.journal.volume
15  
dc.journal.number
3  
dc.journal.pagination
235-240  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Philadelphia  
dc.description.fil
Fil: Bermejo, Emilse. Academia Nacional de Medicina de Buenos Aires; Argentina  
dc.description.fil
Fil: Alberto, Maria Fabiana. Academia Nacional de Medicina de Buenos Aires; Argentina  
dc.description.fil
Fil: Meschengieser, Susana S.. Academia Nacional de Medicina de Buenos Aires; Argentina  
dc.description.fil
Fil: Lazzari, María Ángela. Academia Nacional de Medicina de Buenos Aires; Argentina  
dc.journal.title
Blood Coagulation & Fibrinolysis : An International Journal In Haemostasis And Thrombosis.  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1097/00001721-200404000-00006  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.lww.com/bloodcoagulation/pages/articleviewer.aspx?year=2004&issue=04000&article=00006&type=abstract