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dc.contributor.author
de Winne, Koen
dc.contributor.author
Büscher, Philippe
dc.contributor.author
Luquetti, Alejandro O.
dc.contributor.author
Tavares, Suelene B. N.
dc.contributor.author
Oliveira, Rodrigo A.
dc.contributor.author
Solari, Aldo
dc.contributor.author
Zulantay, Ines
dc.contributor.author
Apt, Werner
dc.contributor.author
Diosque, Patricio
dc.contributor.author
Monje Rumi, Maria Mercedes
dc.contributor.author
Gironés, Nuria
dc.contributor.author
Fresno, Manuel
dc.contributor.author
Lopez Velez, Rogelio
dc.contributor.author
Perez Molina, José A.
dc.contributor.author
Monge Maillo, Begoña
dc.contributor.author
Garcia, Lineth
dc.contributor.author
Deborggraeve, Stijn
dc.date.available
2016-08-11T15:36:13Z
dc.date.issued
2014-01-02
dc.identifier.citation
de Winne, Koen ; Büscher, Philippe ; Luquetti, Alejandro O. ; Tavares, Suelene B. N. ; Oliveira, Rodrigo A. ; et al.; The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study; Public Library Of Science; Neglected Tropical Diseases; 8; 1; 2-1-2014; 2633-2633
dc.identifier.issn
1935-2735
dc.identifier.uri
http://hdl.handle.net/11336/7092
dc.description.abstract
Background: The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU) TcI than for TcII-VI and the test has not been evaluated in chronic Chagas disease patients. Methodology/Principal Findings: We developed a new prototype of the OligoC-TesT based on kinetoplast DNA (kDNA) detection. We evaluated the satDNA and kDNA OligoC-TesTs in a multi-cohort study with 187 chronic Chagas patients and 88 healthy endemic controls recruited in Argentina, Chile and Spain and 26 diseased non-endemic controls from D.R. Congo and Sudan. All specimens were tested in duplicate. The overall specificity in the controls was 99.1% (95% CI 95.2%-99.8%) for the satDNA OligoC-TesT and 97.4% (95% CI 92.6%?99.1%) for the kDNA OligoC-TesT. The overall sensitivity in the patients was 67.9% (95% CI 60.9%-74.2%) for the satDNA OligoC-TesT and 79.1% (95% CI 72.8%-84.4%) for the kDNA OligoC-Test. Conclusions/Significance: Specificities of the two T. cruzi OligoC-TesT prototypes are high on non-endemic and endemic controls. Sensitivities are moderate but significantly (p= 0.0004) higher for the kDNA OligoC-TesT compared to the satDNA OligoC-TesT.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Trypanosoma Cruzi
dc.subject
Diagnostic
dc.subject
Chagas Disease
dc.subject.classification
Parasitología
dc.subject.classification
Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-08-04T18:17:24Z
dc.journal.volume
8
dc.journal.number
1
dc.journal.pagination
2633-2633
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: de Winne, Koen . Institute of Tropical Medicine; Bélgica
dc.description.fil
Fil: Büscher, Philippe . Institute of Tropical Medicine; Bélgica
dc.description.fil
Fil: Luquetti, Alejandro O. . Universidade Federal de Goias; Brasil
dc.description.fil
Fil: Tavares, Suelene B. N. . Universidade Federal de Goias; Brasil
dc.description.fil
Fil: Oliveira, Rodrigo A. . Universidade Federal de Goias; Brasil
dc.description.fil
Fil: Solari, Aldo . Universidad de Santiago de Chile; Chile
dc.description.fil
Fil: Zulantay, Ines . Universidad de Santiago de Chile; Chile
dc.description.fil
Fil: Apt, Werner . Universidad de Santiago de Chile; Chile
dc.description.fil
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
dc.description.fil
Fil: Monje Rumi, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
dc.description.fil
Fil: Gironés, Nuria . Consejo Superior de Investigaciones Cientificas; España
dc.description.fil
Fil: Fresno, Manuel . Consejo Superior de Investigaciones Cientificas; España
dc.description.fil
Fil: Lopez Velez, Rogelio. Ramon y Cajal Hospital, Madrid; España
dc.description.fil
Fil: Perez Molina, José A. . Ramon y Cajal Hospital, Madrid; España
dc.description.fil
Fil: Monge Maillo, Begoña . Ramon y Cajal Hospital, Madrid; España
dc.description.fil
Fil: Garcia, Lineth . Universidad Mayor de San Simon; Bolivia
dc.description.fil
Fil: Deborggraeve, Stijn . Institute of Tropical Medicine; Bélgica
dc.journal.title
Neglected Tropical Diseases
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1371/ journal.pntd.0002633
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/PMC3879245
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/ journal.pntd.0002633
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879245/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0002633
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