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dc.contributor.author
Franci, Tomas
dc.contributor.author
Sanso, Andrea Mariel
dc.contributor.author
Bustamante, Ana Victoria
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Lucchesi, Paula Maria Alejandra
dc.contributor.author
Parma, Alberto Ernesto
dc.date.available
2019-02-21T21:12:58Z
dc.date.issued
2011-09
dc.identifier.citation
Franci, Tomas; Sanso, Andrea Mariel; Bustamante, Ana Victoria; Lucchesi, Paula Maria Alejandra; Parma, Alberto Ernesto; Genetic characterization of non-O157 verocytotoxigenic escherichia coli isolated from raw beef products using multiple-locus variable-number tandem repeat analysis; Mary Ann Liebert; Foodborne Pathogens And Disease; 8; 9; 9-2011; 1019-1023
dc.identifier.issn
1535-3141
dc.identifier.uri
http://hdl.handle.net/11336/70668
dc.description.abstract
Verocytotoxigenic Escherichia coli (VTEC) can produce serious human illness linked to the consumption of contaminated food, mainly of bovine origin. There is growing concern about non-O157 VTEC serotypes, which in some countries cause severe infections in a proportion similar to O157:H7 strains. As several epidemiological studies indicated the important role of meat as the major vehicle in the transmission of this pathogen to human consumers, our aim was to investigate the genetic diversity among non-O157:H7 VTEC isolated from raw beef products. We performed a multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA), and to our knowledge, this is the first time that VTEC serotypes O8:H19, O112:H2, O113:NM, O171:NM, ONT:H7, ONT:H19, and ONT:H21 were typed by this method. MLVA typing grouped the total number of strains from this study (51) into 21 distinct genotypes, and 11 of them were unique. Several MLVA profiles were found in different serotypes, O178:H19 being the most variable. The isolates could be principally discriminated by alleles of three of seven loci studied (CVN001, CVN004, and CVN014), and on the other hand, CVN003 rendered null alleles in all the isolates. As some VNTR markers might be serotype specific, it is possible that the implementation of new VNTR loci will increase intraserotype discrimination.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Mary Ann Liebert
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Verocytotoxigenic Escherichia Coli
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Typing
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Meat
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Non-O157
dc.subject.classification
Otras Ciencias de la Salud
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Ciencias de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Genetic characterization of non-O157 verocytotoxigenic escherichia coli isolated from raw beef products using multiple-locus variable-number tandem repeat analysis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-23T18:54:24Z
dc.journal.volume
8
dc.journal.number
9
dc.journal.pagination
1019-1023
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Franci, Tomas. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina
dc.description.fil
Fil: Sanso, Andrea Mariel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Bustamante, Ana Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Lucchesi, Paula Maria Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Parma, Alberto Ernesto. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Foodborne Pathogens And Disease
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1089/fpd.2010.0814
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/fpd.2010.0814


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