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Artículo

Subtyping of STEC by MLVA in Argentina

Bustamante, Ana VictoriaIcon ; Sanso, Andrea MarielIcon ; Parma, Alberto ErnestoIcon ; Lucchesi, Paula Maria AlejandraIcon
Fecha de publicación: 08/2012
Editorial: Frontiers Research Foundation
Revista: Frontiers in Cellular and Infection Microbiology
ISSN: 2235-2988
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Shiga toxin-producing Escherichia coli (STEC) causes serious human illness such as hemolytic uremic syndrome (HUS). Argentina has the world’s highest rate of this syndrome, which is the leading cause of acute renal failure among children. E. coli O157:H7 is the most common cause of HUS, but a substantial and growing proportion of this illness is caused by infection due to non-O157 strains. Multiple-locus variable-number tandem repeat analysis (MLVA) has become an established technique to subtype STEC. This review will address the use of routine STEC subtyping by MLVA in order to type this group of isolates and to get insight into the genetic diversity of native STEC. With regard to these objectives we modified and adapted two MLVA protocols, one exclusive for O157 and the other, a generic E. coli assay. A total of 202 STEC isolates, from different sources and corresponding to 20 serotypes, have been MLVA genotyped in our laboratory. In our experience, MLVA constitutes a very sensitive tool and enables us to perform an efficient STEC subtyping. The diversity found in many serotypes may be useful for future epidemiological studies of STEC clonality, applied to O157 as well as to non-O157 isolates.
Palabras clave: Stec , Mlva , Genotyping , O157:H7 , Non-O157
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/70667
DOI: http://dx.doi.org/10.3389/fcimb.2012.00111
URL: https://www.frontiersin.org/articles/10.3389/fcimb.2012.00111/full
URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424435/
Colecciones
Articulos(CIVETAN)
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Citación
Bustamante, Ana Victoria; Sanso, Andrea Mariel; Parma, Alberto Ernesto; Lucchesi, Paula Maria Alejandra; Subtyping of STEC by MLVA in Argentina; Frontiers Research Foundation; Frontiers in Cellular and Infection Microbiology; 2; 8-2012; 1-4
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