Artículo
HER2 and ß-catenin protein location: importance in the prognosis of breast cancer patients and their correlation when breast cancer cells suffer stressful situations
Cuello Carrión, Fernando Darío
; Shortrede, Jorge E.; Alvarez Olmedo, Daiana Gisela
; Cayado Gutiérrez, Niubys de Los Milagros
; Castro, Gisela Natalia
; Zoppino, Felipe Carlos Martin
; Guerrero Gimenez, Martin Eduardo
; Martinis, Estefanía Mabel
; Wuilloud, Rodolfo German
; Gomez, Nidia N.; Biaggio, Veronica Silvina
; Orozco, Javier; Gago, Francisco E.; Ciocca, Leonardo A.; Fanelli, Mariel Andrea
; Ciocca, Daniel Ramon
Fecha de publicación:
02/2015
Editorial:
Springer
Revista:
Clinical & Experimental Metastasis
ISSN:
0262-0898
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In human breast cancer, β-catenin localization has been related with disease prognosis. Since HER2-positive patients are an important subgroup, and that in breast cancer cells a direct interaction of β-catenin/HER2 has been reported, in the present study we have explored whether β-catenin location is related with the disease survival. The study was performed in a tumor bank from patients (n = 140) that did not receive specific anti-HER2 therapy. The proteins were detected by immunohistochemistry in serial sections, 47 (33.5 %) patients were HER2-positive with a long follow-up. HER2-positive patients that displayed β-catenin at the plasma membrane (completely surrounding the tumour cells) showed a significant better disease-free survival and overall survival than the patients showing the protein on other locations. Then we explored the dynamics of the co-expression of β-catenin and HER2 in human MCF-7 and SKBR3 cells exposed to different stressful situations. In untreated conditions MCF-7 and SKBR3 cells showed very different β-catenin localization. In MCF-7 cells, cadmium administration caused a striking change in β-catenin localization driving it from plasma membrane to cytoplasmic and perinuclear areas and HER2 showed a similar localization patterns. The changes induced by cadmium were compared with heat shock, H2O2 and tamoxifen treatments. In conclusion, this study shows the dynamical associations of HER2 and β-catenin and their changes in subcellular localizations driven by stressful situations. In addition, we report for the first time the correlation between plasma membrane associated β-catenin in HER2-positive breast cancer and survival outcome, and the importance of the protein localization in breast cancer samples.
Palabras clave:
Breast Cancer
,
Beta Catenin
,
Her2
,
Prognosis
,
Stress
,
Cadmium
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - MENDOZA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MENDOZA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MENDOZA
Articulos(CCT - SAN LUIS)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Articulos(IMBECU)
Articulos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Articulos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Citación
Cuello Carrión, Fernando Darío; Shortrede, Jorge E.; Alvarez Olmedo, Daiana Gisela; Cayado Gutiérrez, Niubys de Los Milagros; Castro, Gisela Natalia; et al.; HER2 and ß-catenin protein location: importance in the prognosis of breast cancer patients and their correlation when breast cancer cells suffer stressful situations; Springer; Clinical & Experimental Metastasis; 32; 2; 2-2015; 151-168
Compartir
Altmétricas