Artículo
Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis
Joray, Mariana Belén
; Trucco, Lucas Daniel
; González, María Laura; Diaz Napal, Georgina Natalia
; Palacios, Sara Maria
; Bocco, Jose Luis
; Carpinella, Maria Cecilia
Fecha de publicación:
12/2015
Editorial:
Hindawi Publishing Corporation
Revista:
Evidence-Based Complementary and Alternative Medicine
ISSN:
1741-427X
e-ISSN:
1741-4288
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The antibacterial and cytotoxic effects of metabolites isolated from an antibacterial extract of Flourensia oolepis were evaluated. Bioguided fractionation led to five flavonoids, identified as 2′,4′-dihydroxychalcone (1), isoliquiritigenin (2), pinocembrin (3), 7-hydroxyflavanone (4), and 7,4′-dihydroxy-3′-methoxyflavanone (5). Compound 1 showed the highest antibacterial effect, with minimum inhibitory concentration (MIC) values ranging from 31 to 62 and 62 to 250 g/mL, against Gram-positive and Gram-negative bacteria, respectively. On further assays, the cytotoxic effect of compounds 1-5 was determined by MTT assay on acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML) cell lines including their multidrug resistant (MDR) phenotypes. Compound 1 induced a remarkable cytotoxic activity toward ALL cells (IC50 = 6.6-9.9 M) and a lower effect against CML cells (IC50 = 27.5-30.0 M). Flow cytometry was used to analyze cell cycle distribution and cell death by PI-labeled cells and by Annexin V/PI staining, respectively. Upon treatment, 1 induced cell cycle arrest in the G2/M phase accompanied by a strong induction of apoptosis. These results describe for the first time the antibacterial metabolites of F. oolepis extract, with 1 being the most effective. This chalcone also emerges as a selective cytotoxic agent against sensitive and resistant leukemic cells, highlighting its potential as a lead compound.
Palabras clave:
Plantas Nativas
,
Citotoxicos Naturales
,
Antibacterianos Naturales
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Joray, Mariana Belén; Trucco, Lucas Daniel; González, María Laura; Diaz Napal, Georgina Natalia; Palacios, Sara Maria; et al.; Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis; Hindawi Publishing Corporation; Evidence-Based Complementary and Alternative Medicine; 2015; 12-2015; 1-11; 912484
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