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dc.contributor.author
Fabro, Georgina  
dc.contributor.author
Rovasio, Roberto Americo  
dc.contributor.author
Civalero, Silvia  
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Frenkel, Anat  
dc.contributor.author
Caplan, S. Roy  
dc.contributor.author
Eisenbach, Michael  
dc.contributor.author
Giojalas, Laura Cecilia  
dc.date.available
2019-02-18T20:10:34Z  
dc.date.issued
2002-11-01  
dc.identifier.citation
Fabro, Georgina; Rovasio, Roberto Americo; Civalero, Silvia; Frenkel, Anat; Caplan, S. Roy; et al.; Chemotaxis of Capacitated Rabbit Spermatozoa to Follicular Fluid Revealed by a Novel Directionality-Based Assay; Society for the Study of Reproduction; Biology of Reproduction; 67; 5; 1-11-2002; 1565-1571  
dc.identifier.issn
0006-3363  
dc.identifier.uri
http://hdl.handle.net/11336/70397  
dc.description.abstract
Precontact communication between gametes is established by chemotaxis. Sperm chemotaxis toward factor(s) in follicular fluid (FF) has been demonstrated in humans and mice. In humans, the chemotactic responsiveness is restricted to capacitated spermatozoa. Here, we investigated whether sperm chemotaxis to factor(s) present in FF also occurs in rabbits and, if so, whether only capacitated spermatozoa are chemotactically responsive. Chemotaxis assays were performed by videomicroscopy in a Zigmond chamber. We measured chemotactic responsiveness as a function of FF dilution by means of a novel directionality-based method that considers the ratio between the distances traveled by the spermatozoa both parallel to the chemoattractant gradient and perpendicular to it. A peak of maximal response was observed at 10-4 dilution of FF, resulting in a typical chemotactic concentration-dependent curve in which 23% of the spermatozoa were chemotactically responsive. In contrast, the percentage of cells exhibiting FF-dependent enhanced speed of swimming increased with the FF concentration, whereas the percentage of cells maintaining linear motility decreased with the FF concentration. The percentages of chemotactically responsive cells were very similar to those of capacitated spermatozoa. Depletion of the latter by stimulation of the acrosome reaction resulted in a total loss of the chemotactic response, whereas the reappearance of capacitated cells resulted in a recovery of chemotactic responsiveness. We conclude that rabbit spermatozoa, like human spermatozoa, are chemotactically responsive to FF factor(s) and acquire this responsiveness as part of the capacitation process.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Society for the Study of Reproduction  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Acrosome Reaction  
dc.subject
Fertilization  
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Gamete Biology  
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Sperm Capacitation  
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Sperm Motility And Transport  
dc.subject.classification
Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Chemotaxis of Capacitated Rabbit Spermatozoa to Follicular Fluid Revealed by a Novel Directionality-Based Assay  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-11-12T13:46:34Z  
dc.identifier.eissn
1529-7268  
dc.journal.volume
67  
dc.journal.number
5  
dc.journal.pagination
1565-1571  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Madison  
dc.description.fil
Fil: Fabro, Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina  
dc.description.fil
Fil: Rovasio, Roberto Americo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina  
dc.description.fil
Fil: Civalero, Silvia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina  
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Fil: Frenkel, Anat. Weizmann Institute of Science; Israel  
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Fil: Caplan, S. Roy. McGill University; Canadá. Weizmann Institute of Science; Israel  
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Fil: Eisenbach, Michael. Weizmann Institute of Science; Israel  
dc.description.fil
Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina  
dc.journal.title
Biology of Reproduction  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1095/biolreprod.102.006395  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/67/5/1565/2683774