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dc.contributor.author
Garay, Fernando Sebastian
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dc.contributor.author
Kisiel, Greggory
dc.contributor.author
Fang, Aiping
dc.contributor.author
Lindner, Erno
dc.date.available
2019-02-18T14:52:19Z
dc.date.issued
2010-07
dc.identifier.citation
Garay, Fernando Sebastian; Kisiel, Greggory; Fang, Aiping; Lindner, Erno; Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 397; 5; 7-2010; 1873-1881
dc.identifier.issn
1618-2642
dc.identifier.uri
http://hdl.handle.net/11336/70325
dc.description.abstract
This article presents a simple chronoamperometric immunosensor for the quantitative assessment of creatine kinase MB (CK-MB) in 50 μL undiluted serum samples. The immunosensor consists of gold working and counter electrodes patterned onto a glass chip by thin-film photolithography and an external Ag|AgCl reference electrode. The detection limit (DL) of the chronoamperometric method is 13 ng mL-1 (DL = 2×RMSD/S, where RMSD is the residual mean standard deviation of the measured points around a calibration curve with a slope of S). In spiked serum samples, the response was linear up to 300 ng mL-1 of CK-MB. A surface plasmon resonance (SPR) system with simultaneous electrochemical detection (EC-SPR) aided the development of the sandwich immunoassay. Real-time monitoring of the SPR signal was used to optimize the capture antibody immobilization, CK-MB and detection antibody binding, as well as to minimize the nonspecific adsorption of serum proteins to the sensor surface. The detection antibody has been labeled with alkaline phosphatase (ALP) enzyme for sensitive electrochemical detection. ALP catalyzes the hydrolysis of ascorbic acid phosphate and generates ascorbic acid, which is measured chronoamperometrically. The electrochemical immunoassay for CK-MB was less sensitive to nonspecific adsorption related interferences, had a better detection limit, and required a lower volume of sample than the SPR method. © 2010 Springer-Verlag.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Heidelberg
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Creatine Kinase Mb
dc.subject
Electrochemistry
dc.subject
Immunosensor
dc.subject
Serum
dc.subject
Spr
dc.subject.classification
Otras Ciencias Químicas
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-02-12T17:06:37Z
dc.identifier.eissn
1618-2650
dc.journal.volume
397
dc.journal.number
5
dc.journal.pagination
1873-1881
dc.journal.pais
Alemania
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dc.journal.ciudad
Heidelberg
dc.description.fil
Fil: Garay, Fernando Sebastian. University of Memphis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Kisiel, Greggory. University of Memphis; Estados Unidos. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Fang, Aiping. University of Memphis; Estados Unidos
dc.description.fil
Fil: Lindner, Erno. University of Memphis; Estados Unidos
dc.journal.title
Analytical and Bioanalytical Chemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.springerlink.com/content/u30m0860h40mu62m/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00216-010-3736-2
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