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dc.contributor.author
Alvarez, Yanina Daniela
dc.contributor.author
Belingheri, Ana Verónica
dc.contributor.author
Perez Bay, Andrés Ezequiel
dc.contributor.author
Javis, Scott E.
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Tedford, H. William
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Zamponi, Gerald
dc.contributor.author
Marengo, Fernando Diego
dc.date.available
2015-06-11T20:33:59Z
dc.date.issued
2013-01
dc.identifier.citation
Alvarez, Yanina Daniela; Belingheri, Ana Verónica; Perez Bay, Andrés Ezequiel; Javis, Scott E.; Tedford, H. William; et al.;The immediately releasable pool of mouse chromaffin cell vesicles is coupled to P/Q-type calcium channels via the synaptic protein interaction site; Public Library Science; Plos One; 8; 1; 1-2013; e054846
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/699
dc.description.abstract
It is generally accepted that the immediately releasable pool is a group of readily releasable vesicles that are closely associated with voltage dependent Ca(2+) channels. We have previously shown that exocytosis of this pool is specifically coupled to P/Q Ca(2+) current. Accordingly, in the present work we found that the Ca(2+) current flowing through P/Q-type Ca(2+) channels is 8 times more effective at inducing exocytosis in response to short stimuli than the current carried by L-type channels. To investigate the mechanism that underlies the coupling between the immediately releasable pool and P/Q-type channels we transiently expressed in mouse chromaffin cells peptides corresponding to the synaptic protein interaction site of Cav2.2 to competitively uncouple P/Q-type channels from the secretory vesicle release complex. This treatment reduced the efficiency of Ca(2+) current to induce exocytosis to similar values as direct inhibition of P/Q-type channels via ω-agatoxin-IVA. In addition, the same treatment markedly reduced immediately releasable pool exocytosis, but did not affect the exocytosis provoked by sustained electric or high K(+) stimulation. Together, our results indicate that the synaptic protein interaction site is a crucial factor for the establishment of the functional coupling between immediately releasable pool vesicles and P/Q-type Ca(2+) channels.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Irp
dc.subject
P/Q-Type Ca(2+) Channels
dc.subject
Synprint
dc.subject
Chromaffin Cells
dc.subject.classification
Ciencias Naturales y Exactas
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Ciencias Biológicas
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Otras Ciencias Biológicas
dc.title
The immediately releasable pool of mouse chromaffin cell vesicles is coupled to P/Q-type calcium channels via the synaptic protein interaction site
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
8
dc.journal.number
1
dc.journal.pagination
e054846
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Alvarez, Yanina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina;
dc.description.fil
Fil: Belingheri, Ana Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina;
dc.description.fil
Fil: Perez Bay, Andrés Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina;
dc.description.fil
Fil: Javis, Scott E.. University of Calgary. Hotchkiss Brain Institute; Canada;
dc.description.fil
Fil: Tedford, H. William. University of Calgary. Hotchkiss Brain Institute; Canada;
dc.description.fil
Fil: Zamponi, Gerald. University of Calgary. Hotchkiss Brain Institute; Canada;
dc.description.fil
Fil: Marengo, Fernando Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina;
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0054846
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