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dc.contributor.author Ostermann Porcel, María Victoria
dc.contributor.author Vizoso Pinto, María Guadalupe
dc.contributor.author Haase, Rudolf
dc.contributor.author Nitschko, Hans
dc.contributor.author Jaeger, Simone
dc.contributor.author Sander, Michaela
dc.contributor.author Motz, Manfred
dc.contributor.author Mohn, Ulrich
dc.contributor.author Baiker, Armin
dc.date.available 2019-02-11T21:59:03Z
dc.date.issued 2012-01
dc.identifier.citation Ostermann Porcel, María Victoria; Vizoso Pinto, María Guadalupe; Haase, Rudolf; Nitschko, Hans; Jaeger, Simone; et al.; Systematic screening for novel, serologically reactive Hepatitis e Virus epitopes; BioMed Central; Virology Journal; 9; 1-2012; 1-9
dc.identifier.issn 1743-422X
dc.identifier.uri http://hdl.handle.net/11336/69950
dc.description.abstract Background: The National Institutes of Health classified Hepatitis E as an emerging disease since Hepatitis E Virus (HEV) is the major cause of acute hepatitis in developing countries. Interestingly, an increasing number of sporadic cases of HEV infections are described in industrialized countries as zoonosis from domestic livestock. Despite the increasing relevance of this pathogen in clinical virology, commercial antibody assays are mainly based on fragments of HEV open reading frame (ORF) 2 and ORF3. The largest ORF1 (poly-)protein, however, is not part of current testing formats. Methods. From a synthesized full length HEV genotype 1 cDNA-bank we constructed a complete HEV gene library consisting of 15 respective HEV ORF domains. After bacterial expression and purification of nine recombinant HEV proteins under denaturating conditions serum profiling experiments using 55 sera from patients with known infection status were performed in microarray format. SPSS software assessed the antigenic potential of these nine ORF domains in comparison to seven commercial HEV antigens (genotype 1 and 3) by performing receiver operator characteristics, logistic regression and correlation analysis. Results: HEV antigens produced with our method for serum profiling experiments exhibit the same quality and characteristics as commercial antigens. Serum profiling experiments detected Y, V and X domains as ORF1-antigens with potentially comparable diagnostic significance as the well established epitopes of ORF2 and ORF3. However no obvious additional increase in sensitivity or specificity was achieved in diagnostic testing as revealed by bioinformatic analysis. Additionally we found that the C-terminal domain of the potential transmembrane protein ORF3 is responsible for IgG and IgM seroreactivity. Data suggest that there might be a genotype specific seroreactivity of homologous ORF2-antigens. Conclusions: The diagnostic value of identified ORF1 epitopes might not necessarily improve sensitivity and specificity, but broaden the overall quality of existing test systems. ORF2 and ORF3-antigens are still commonly used in diagnostic assays and possibly hold the potential to serologically differentiate between genotype 1 and 3 infections. Our systematic approach is a suitable method to investigate HEV domains for their serologic antigenicity. Epitope screening of native viral domains could be a preferable tool in developing new serologic test components.
dc.format application/pdf
dc.language.iso eng
dc.publisher BioMed Central
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject HEPATITIS E VIRUS
dc.subject GENOMW-WIDE
dc.subject SEROLOGY
dc.subject VIRAL ANTIGENS
dc.subject DIAGNOSTIC TEST DEVELOPMENT
dc.subject.classification Inmunología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Systematic screening for novel, serologically reactive Hepatitis e Virus epitopes
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2019-01-14T15:04:06Z
dc.journal.volume 9
dc.journal.pagination 1-9
dc.journal.pais Reino Unido
dc.journal.ciudad Londres
dc.description.fil Fil: Ostermann Porcel, María Victoria. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
dc.description.fil Fil: Vizoso Pinto, María Guadalupe. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil Fil: Haase, Rudolf. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania
dc.description.fil Fil: Nitschko, Hans. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania
dc.description.fil Fil: Jaeger, Simone. Mikrogen GmbH; Alemania
dc.description.fil Fil: Sander, Michaela. Mikrogen Gmbh; Alemania
dc.description.fil Fil: Motz, Manfred. Mikrogen Gmbh; Alemania
dc.description.fil Fil: Mohn, Ulrich. Mikrogen Gmbh; Alemania
dc.description.fil Fil: Baiker, Armin. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute. Cátedra Virology; Alemania. Bavarian Health and Food Safety Authority; Alemania
dc.journal.title Virology Journal
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.virologyj.com/content/9/1/28/abstract
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1186/1743-422X-9-28
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-28
dc.conicet.fuente individual


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)