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dc.contributor.author Baravalle, María Eugenia
dc.contributor.author Thompson, Carolina Soledad
dc.contributor.author Torioni de Echaide, S.
dc.contributor.author Palacios, Carlos Adolfo
dc.contributor.author Valentini, B.
dc.contributor.author Suárez, C. E.
dc.contributor.author Christensen, M. Florín
dc.contributor.author Echaide, Ignacio Eduardo
dc.date.available 2019-02-06T22:52:52Z
dc.date.issued 2010-12
dc.identifier.citation Baravalle, María Eugenia; Thompson, Carolina Soledad; Torioni de Echaide, S.; Palacios, Carlos Adolfo; Valentini, B.; et al.; The novel protein BboRhop68 is expressed by intraerythrocytic stages of Babesia bovis; Elsevier Ireland; Parasitology International; 59; 4; 12-2010; 571-578
dc.identifier.issn 1383-5769
dc.identifier.uri http://hdl.handle.net/11336/69615
dc.description.abstract The apical complex of intracellular hemoparasites contains organelles like micronemes and rhoptries, specialized structures required for adherence and invasion of host cells. Several molecules discharged from rhoptries have been identified from Plasmodium spp., but only a single rhoptry associated protein-1 (RAP-1) has been characterized from Babesia bovis. In silico search of the B. bovis genome allowed to identifying a sequence homologous to the gene that encodes a P. falciparum rhoptry protein PfRhop148. The intron-less 1830. bp novel gene, predicted a 68. kDa protein, and it was highly conserved among different B. bovis strains and isolates. The deducted protein from the B. bovis T2Bo strain, named BboRhop68, showed two putative transmembrane domains, at least seven B-cell epitopes, and a well conserved DUF501 super family domain. The bborhop68 gene was amplified, analyzed and compared among different B. bovis strains and isolates showing overall high sequence conservation. A fragment of bborhop68 was expressed as a recombinant fusion protein (rBboRhop68). The mice anti-rBboRhop68 serum identified the novel protein in intraerythrocytic trophozoites and merozoites by WB and ELISA, but not in free merozoites. Sera from naturally and experimentally infected bovines also recognized BboRhop68, suggesting that it is expressed and immunogenic during B. bovis infection. Fluorescence microscopy analysis using anti-rBboRhop68 antibodies showed a rod structure associated to trophozoites and merozoites infected erythrocytes, but this pattern of reactivity was not observed in free merozoites. The BboRhop68 was also not detected in ELISA based on solubilized merozoites. Thus, at least three independent lines of evidence support differential expression of BboRhop68 in intraerythrocytic stages of B. bovis and its possible functional role immediately after B. bovis erythrocyte invasion. The results of this work suggest that BboRhop68 could be considered as a novel additional target for developing improved methods to control bovine babesiosis.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Ireland
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject APICOMPLEXA
dc.subject BABESIA BOVIS
dc.subject BBORHOP68
dc.subject RHOPTRY
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title The novel protein BboRhop68 is expressed by intraerythrocytic stages of Babesia bovis
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2019-01-29T18:09:03Z
dc.journal.volume 59
dc.journal.number 4
dc.journal.pagination 571-578
dc.journal.pais Irlanda
dc.journal.ciudad Shannon
dc.description.fil Fil: Baravalle, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.fil Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.fil Fil: Torioni de Echaide, S.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.fil Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina
dc.description.fil Fil: Valentini, B.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.fil Fil: Suárez, C. E.. United States Department of Agriculture. Agricultural Research Service; Argentina
dc.description.fil Fil: Christensen, M. Florín. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
dc.journal.title Parasitology International
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1383576910001315
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.parint.2010.07.008
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)