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dc.contributor.author
Baptista, Juliana Cristina
dc.contributor.author
Machado, Marcos Antonio
dc.contributor.author
Homem, Rafael Augusto
dc.contributor.author
Torres, Pablo Sebastian
dc.contributor.author
Vojnov, Adrián Alberto
dc.contributor.author
do Amaral, Alexandre Morais
dc.date.available
2019-01-31T16:49:30Z
dc.date.issued
2010-03
dc.identifier.citation
Baptista, Juliana Cristina; Machado, Marcos Antonio; Homem, Rafael Augusto; Torres, Pablo Sebastian; Vojnov, Adrián Alberto; et al.; Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 33; 1; 3-2010; 146-153
dc.identifier.issn
1415-4757
dc.identifier.uri
http://hdl.handle.net/11336/69112
dc.description.abstract
The Gram-negative bacterium Xanthomonas axonopodispv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xaccapacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodispv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsDgene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodispv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sociedade Brasileira de Genética
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Citrus Canker
dc.subject
Transposome
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Type Ii Secretion System
dc.subject.classification
Agricultura
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Agricultura, Silvicultura y Pesca
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CIENCIAS AGRÍCOLAS
dc.title
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-29T18:08:27Z
dc.identifier.eissn
1678-4685
dc.journal.volume
33
dc.journal.number
1
dc.journal.pagination
146-153
dc.journal.pais
Brasil
dc.journal.ciudad
San Pablo
dc.description.fil
Fil: Baptista, Juliana Cristina. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; Brasil
dc.description.fil
Fil: Machado, Marcos Antonio. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil
dc.description.fil
Fil: Homem, Rafael Augusto. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; Brasil
dc.description.fil
Fil: Torres, Pablo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
dc.description.fil
Fil: Vojnov, Adrián Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
dc.description.fil
Fil: do Amaral, Alexandre Morais. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
dc.journal.title
Genetics and Molecular Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1590/S1415-47572009005000110
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/z5c56j
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036071/


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