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dc.contributor.author García, Paula
dc.contributor.author Ramallo, Ivana Ayelen
dc.contributor.author Furlan, Ricardo Luis Eugenio
dc.date.available 2019-01-30T14:19:13Z
dc.date.issued 2016-12
dc.identifier.citation García, Paula; Ramallo, Ivana Ayelen; Furlan, Ricardo Luis Eugenio; Reverse phase compatible TLC-bioautography for detection of tyrosinase inhibitors; John Wiley & Sons Ltd; Phytochemical Analysis; 28; 2; 12-2016; 101-105
dc.identifier.issn 0958-0344
dc.identifier.uri http://hdl.handle.net/11336/68961
dc.description.abstract Introduction Reverse phase chromatography and bioautographic assays are key tools for natural product bioguided isolation; however, their direct coupling has not been fully achieved. Objectives To develop a bioautographic assay to detect tyrosinase inhibitors present in complex matrices sorbed on reverse phase (RP) TLC-plates that can be used for bioguided isolation of bioactive compounds. Methods Enzyme gel entrapment with an amphiphilic copolymer was used for assay development. The gel turns into a brown skin like colour due to tyrosinase catalysed oxidation of L-tyrosine. The inhibitors are visualised as clear spots against a brown coloured background. Results The assay was able to localise cinnamaldehyde in Cinnamomum cassia essential oil, as its main constituent with known tyrosinase inhibition properties. The assay allowed the detection of 0.03% (w/w) of kojic acid co-spotted with a methanolic extract of Sphaeralcea bonariensis and chromatographed on RP-TLC.Conclusion The developed assay is able to detect, with high sensitivity, tyrosinase inhibitors present in complex matrices that were chromatographed in RP-TLC. Results can be easily read by colour change, inhibitors appear as clear spots in a darker background.
dc.format application/pdf
dc.language.iso eng
dc.publisher John Wiley & Sons Ltd
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject TLC-BIOAUTOGRAPHY
dc.subject HERBAL DRUGS
dc.subject TYROSINASE INHIBITION
dc.subject DERMATOLOGICAL DISORDERS
dc.subject.classification Otras Ciencias Químicas
dc.subject.classification Ciencias Químicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Reverse phase compatible TLC-bioautography for detection of tyrosinase inhibitors
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-12-19T13:21:07Z
dc.identifier.eissn 1099-1565
dc.journal.volume 28
dc.journal.number 2
dc.journal.pagination 101-105
dc.journal.pais Reino Unido
dc.journal.ciudad Londres
dc.description.fil Fil: García, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario. Universidad Nacional de Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario; Argentina
dc.description.fil Fil: Ramallo, Ivana Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario. Universidad Nacional de Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario; Argentina
dc.description.fil Fil: Furlan, Ricardo Luis Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario. Universidad Nacional de Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario; Argentina
dc.journal.title Phytochemical Analysis
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/pca.2655
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.2655
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  • Articulos (IIDEFAR) [1]
    Articulos de INSTITUTO DE INVESTIGACIONES PARA EL DESCUBRIMIENTO DE FARMACOS DE ROSARIO

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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)