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dc.contributor.author
García, Paula
dc.contributor.author
Ramallo, Ivana Ayelen
dc.contributor.author
Furlan, Ricardo Luis Eugenio
dc.date.available
2019-01-30T14:19:13Z
dc.date.issued
2016-12
dc.identifier.citation
García, Paula; Ramallo, Ivana Ayelen; Furlan, Ricardo Luis Eugenio; Reverse phase compatible TLC-bioautography for detection of tyrosinase inhibitors; John Wiley & Sons Ltd; Phytochemical Analysis; 28; 2; 12-2016; 101-105
dc.identifier.issn
0958-0344
dc.identifier.uri
http://hdl.handle.net/11336/68961
dc.description.abstract
Introduction Reverse phase chromatography and bioautographic assays are key tools for natural product bioguided isolation; however, their direct coupling has not been fully achieved. Objectives To develop a bioautographic assay to detect tyrosinase inhibitors present in complex matrices sorbed on reverse phase (RP) TLC-plates that can be used for bioguided isolation of bioactive compounds. Methods Enzyme gel entrapment with an amphiphilic copolymer was used for assay development. The gel turns into a brown skin like colour due to tyrosinase catalysed oxidation of L-tyrosine. The inhibitors are visualised as clear spots against a brown coloured background. Results The assay was able to localise cinnamaldehyde in Cinnamomum cassia essential oil, as its main constituent with known tyrosinase inhibition properties. The assay allowed the detection of 0.03% (w/w) of kojic acid co-spotted with a methanolic extract of Sphaeralcea bonariensis and chromatographed on RP-TLC.Conclusion The developed assay is able to detect, with high sensitivity, tyrosinase inhibitors present in complex matrices that were chromatographed in RP-TLC. Results can be easily read by colour change, inhibitors appear as clear spots in a darker background.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Tlc-Bioautography
dc.subject
Herbal Drugs
dc.subject
Tyrosinase Inhibition
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Dermatological Disorders
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Otras Ciencias Químicas
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Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Reverse phase compatible TLC-bioautography for detection of tyrosinase inhibitors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-12-19T13:21:07Z
dc.identifier.eissn
1099-1565
dc.journal.volume
28
dc.journal.number
2
dc.journal.pagination
101-105
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: García, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario. Universidad Nacional de Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario; Argentina
dc.description.fil
Fil: Ramallo, Ivana Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario. Universidad Nacional de Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario; Argentina
dc.description.fil
Fil: Furlan, Ricardo Luis Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario. Universidad Nacional de Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario; Argentina
dc.journal.title
Phytochemical Analysis
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/pca.2655
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.2655


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    Articulos de INSTITUTO DE INVESTIGACIONES PARA EL DESCUBRIMIENTO DE FARMACOS DE ROSARIO

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