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dc.contributor.author
Bonfiglio, Juan José
dc.contributor.author
Maccarrone, Giuseppina
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Rewerts, Christiane
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Holsboer, Florian
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Arzt, Eduardo Simon
dc.contributor.author
Turck, Christoph W.
dc.contributor.author
Silberstein Cuña, Susana Iris
dc.date.available
2019-01-29T19:53:19Z
dc.date.issued
2011-02
dc.identifier.citation
Bonfiglio, Juan José; Maccarrone, Giuseppina; Rewerts, Christiane; Holsboer, Florian; Arzt, Eduardo Simon; et al.; Characterization of the B-Raf interactome in mouse hippocampal neuronal cells; Elsevier Science; Journal Of Proteomics; 74; 2; 2-2011; 186-198
dc.identifier.issn
1874-3919
dc.identifier.uri
http://hdl.handle.net/11336/68884
dc.description.abstract
B-Raf links a variety of extracellular stimuli downstream of cell surface receptors, constituting a determining factor in the ability of neurons to activate ERK. A detailed study of the B-Raf interactome is necessary to clarify the intricacy of B-Raf-dependent signal transduction. We used a mouse hippocampal cell line (HT22) that expresses B-Raf at high levels, to identify B-Raf associated proteins under endogenous expression conditions, avoiding artificial interactions from overexpression studies. We used stringent procedures to co-immunoprecipitate proteins that specifically associate with endogenous B-Raf with the help of gel electrophoresis separation and off-line LC-MALDI-MS/MS proteomic analysis. Our stringent protein identification criteria allowed confident identification of B-Raf interacting proteins under non-stimulating conditions. The presence of previously reported B-Raf interactors among the list of proteins identified confirms the quality of proteomic data. We identified tubulin and actin as B-Raf interactors for the first time, among structural and accessory proteins of cell cytoskeleton, molecular chaperones (Hsc70, GRP78), and cellular components involved in aspects of mRNA metabolism and translation. Interactions were validated in HT22 cells and in the neuronal cell line Neuro-2a providing further evidence that the identified proteins are B-Raf interactors, which constitute a basis for understanding MAPK pathway regulation in neurons.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
B-Raf
dc.subject
Interactome
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Mass Spectrometry
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Neurons
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Proteomics
dc.title
Characterization of the B-Raf interactome in mouse hippocampal neuronal cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-29T18:07:15Z
dc.journal.volume
74
dc.journal.number
2
dc.journal.pagination
186-198
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Bonfiglio, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
dc.description.fil
Fil: Maccarrone, Giuseppina. Max Planck Institute of Psychiatry; Alemania
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Fil: Rewerts, Christiane. Max Planck Institute of Psychiatry; Alemania
dc.description.fil
Fil: Holsboer, Florian. Max Planck Institute of Psychiatry; Alemania
dc.description.fil
Fil: Arzt, Eduardo Simon. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
dc.description.fil
Fil: Turck, Christoph W.. Max Planck Institute of Psychiatry; Alemania
dc.description.fil
Fil: Silberstein Cuña, Susana Iris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
dc.journal.title
Journal Of Proteomics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jprot.2010.10.006
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1874391910002940