Artículo

Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation

Yushchenko, Dmytro A.; Fauerbach, Jonathan ArturoIcon ; Thirunavukkuarasu, Shyamala; Jares, Elizabeth AndreaIcon ; Jovin, Thomas M.
Fecha de publicación: 06/2010
Editorial: American Chemical Society
Revista: Journal of the American Chemical Society
ISSN: 0002-7863
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of α-synuclein aggregation, a process associated with Parkinsons disease.
Palabras clave: Polarity Sensitive Dyes , Amyloid , Synuclein
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/68821
DOI: http://dx.doi.org/10.1021/ja102838n
URL: https://pubs.acs.org/doi/abs/10.1021/ja102838n
Colecciones
Articulos(CIHIDECAR)
Articulos de CENTRO DE INVESTIGACIONES EN HIDRATOS DE CARBONO
Citación
Yushchenko, Dmytro A.; Fauerbach, Jonathan Arturo; Thirunavukkuarasu, Shyamala; Jares, Elizabeth Andrea; Jovin, Thomas M.; Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation; American Chemical Society; Journal of the American Chemical Society; 132; 23; 6-2010; 7860-7861
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