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dc.contributor.author
Víctor, M.
dc.contributor.author
Pizones Ruiz Henestrosa, Víctor Manuel
dc.contributor.author
Martinez, María Julia
dc.contributor.author
Patino, Juan M. R.
dc.contributor.author
Pilosof, Ana Maria Renata
dc.date.available
2019-01-18T19:30:18Z
dc.date.issued
2012-07
dc.identifier.citation
Víctor, M.; Pizones Ruiz Henestrosa, Víctor Manuel; Martinez, María Julia; Patino, Juan M. R.; Pilosof, Ana Maria Renata; A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions; American Oil Chemists' Society; Journal of the American Oil Chemists Society; 89; 7; 7-2012; 1183-1191
dc.identifier.issn
0003-021X
dc.identifier.uri
http://hdl.handle.net/11336/68268
dc.description.abstract
In this work, the complex assembly of one of the major storage proteins in soybean, glycinin, was analyzed using dynamic light scattering, from the hydrodynamic diameter of assembled forms in solution. The protein concentration and temperature were maintained constant at 10-1% w/w and 20 °C, respectively, and the pH was 7.6, 7.0 and 3.0. By analyzing the intensity and volume size distributions, a complex equilibrium between self-assembled forms could be determined. At pH 7.6 and an ionic strength of 0.5 M, where the self-assembly of glycinin has been widely reported in the literature, the DLS technique revealed an equilibrium between different assembled forms, that shifted towards the 11S form. At a lower ionic strength for pH 3.0 or 7.0, the 7S form predominated. The hydrodynamic diameter evolved differently upon heating, depending on pH and ionic strength. For pH 7 (I = 0.05) and 7.6 (I = 0.5) a significant increase in d H was observed at a temperatures of 55 and 70 °C, respectively, which were significantly lower than the denaturation onset temperatures as determined by DSC. No changes in dH nor a transition endotherm were observed at pH 3.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Oil Chemists' Society
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Dynamic Light Scattering
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Globulin
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Soy
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Structure
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Otras Ingeniería Química
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Ingeniería Química
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-16T18:28:54Z
dc.journal.volume
89
dc.journal.number
7
dc.journal.pagination
1183-1191
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Víctor, M.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
dc.description.fil
Fil: Pizones Ruiz Henestrosa, Víctor Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Sevilla; España
dc.description.fil
Fil: Martinez, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
dc.description.fil
Fil: Patino, Juan M. R.. Universidad de Sevilla; España
dc.description.fil
Fil: Pilosof, Ana Maria Renata. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina
dc.journal.title
Journal of the American Oil Chemists Society
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11746-012-2029-7
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://aocs.onlinelibrary.wiley.com/doi/abs/10.1007/s11746-012-2029-7