Mostrar el registro sencillo del ítem

dc.contributor.author
Ladelfa, Maria Fatima
dc.contributor.author
del Medico Zajac, Maria Paula
dc.contributor.author
Kotsias, Fiorella
dc.contributor.author
Delgado, Fernando Oscar
dc.contributor.author
Muylkens, Benoît
dc.contributor.author
Thiry, Julien
dc.contributor.author
Thiry, Etienne
dc.contributor.author
Romera, Sonia
dc.date.available
2019-01-18T17:51:57Z
dc.date.issued
2011-06
dc.identifier.citation
Ladelfa, Maria Fatima; del Medico Zajac, Maria Paula; Kotsias, Fiorella; Delgado, Fernando Oscar; Muylkens, Benoît; et al.; Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains; BioMed Central; Acta Veterinaria Scandinavica; 53; 1; 6-2011; 1-8
dc.identifier.issn
0044-605X
dc.identifier.uri
http://hdl.handle.net/11336/68255
dc.description.abstract
Background: Bovine herpesvirus 5 (BoHV-5) is an alphaherpesvirus responsible for meningoencephalitis in young cattle and it is antigenically and genetically related to bovine herpesvirus 1. BoHV-5 outbreaks are sporadic and restricted in their geographical distribution, being mostly detected in the Southern hemisphere. The N569 and A663 strains are prototypes of the a and b subtypes of BoHV-5, however, scarce information about their in vitro and in vivo properties is currently available.Methods: For the in vitro comparison between BoHV-5 A663 and N569 strains, viral growth kinetics, lysis and infection plaque size assays were performed. Additionally, an experimental infection of cattle with BoHV-5 A663 and N569 strains was carried out. Viral excretion, development of neurological signs, presence of specific antibodies in serum and nasal swabs and presence of latent BoHV-5 DNA in trigeminal ganglion, were analyzed. Histopathological examination of samples belonging to inoculated animals was also performed.Results: The lytic capacity and the cell-to-cell spread was lower for the A663 strain compared to the N569 strain, however, the production of total infectious viral particles was similar between both strains. Concerning the in vivo properties, the A663 and N569 strains are able to induce similar degrees of pathogenicity in cattle.Conclusions: Our results show that the A663 strain used in this study is less adapted to in vitro replication in MDBK cells than the N569 strain and, although slight differences were observed, both strains are able to induce a similar degree of virulence in the natural host.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bovine Herpesvirus 5
dc.subject
A663 And N569 Strain
dc.subject
Growth Kinetcis
dc.subject
Infection And Latency in Bovines
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-17T14:10:59Z
dc.journal.volume
53
dc.journal.number
1
dc.journal.pagination
1-8
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Ladelfa, Maria Fatima. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: del Medico Zajac, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Kotsias, Fiorella. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Muylkens, Benoît. Université de Liège; Bélgica
dc.description.fil
Fil: Thiry, Julien. Université de Liège; Bélgica
dc.description.fil
Fil: Thiry, Etienne. Université de Liège; Bélgica
dc.description.fil
Fil: Romera, Sonia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Acta Veterinaria Scandinavica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://actavetscand.biomedcentral.com/articles/10.1186/1751-0147-53-37
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/1751-0147-53-37


Archivos asociados

 

Comunidades y colecciones

Mostrar el registro sencillo del ítem