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dc.contributor.author
Grynberg, Michaël
dc.contributor.author
Pierre, Alice
dc.contributor.author
Rey, Rodolfo Alberto
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dc.contributor.author
Leclerc, Arnaud
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Arouche, Nassim
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Hesters, Laetitia
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Catteau Jonard, Sophie
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Frydman, René
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Picard, Jean Yves
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Fanchin, Renato
dc.contributor.author
Veitia, Reiner
dc.contributor.author
Di Clemente, Nathalie
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Taieb, Joëlle
dc.date.available
2019-01-16T17:51:07Z
dc.date.issued
2012-09
dc.identifier.citation
Grynberg, Michaël; Pierre, Alice; Rey, Rodolfo Alberto; Leclerc, Arnaud; Arouche, Nassim; et al.; Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors; Endocrine Society; Journal of Clinical Endocrinology and Metabolism; 97; 9; 9-2012; 1649-1657
dc.identifier.issn
0021-972X
dc.identifier.uri
http://hdl.handle.net/11336/68131
dc.description.abstract
Background: Anti-Müllerian hormone (AMH) is a member of the TGF-β family, which limits follicle maturation. Recently serum AMH has been recognized as a useful diagnostic and prognostic tool in human reproductive endocrinology. Objective: The aim of this study was to investigate the regulation of human ovarian AMH by estradiol and FSH. Methods: AMH mRNA were quantified by real time RT-PCR in human granulosa cells (GC). AMH transcription was studied in KK1 GC cotransfected with estrogen receptors (ER)-β or ERα, and normal human AMH promoter-luciferase construct (hAMH-luc) or mutated AMH promoter reporter constructs. Binding sites for estradiol (estrogen response element half-site) and steroidogenic factor 1 were disrupted by targeted mutagenesis. The level of ER in GC was determined by quantitative RT-PCR and Western blotting. Results: In KK1 cells, estradiol up-regulated and inhibited hAMH-luc in the presence of ERα and ERβrespectively. Disruption of estrogen response element half-site and/or steroidogenic factor 1 binding sites did not modify ERβ-mediated effect of estradiol on hAMH-luc, whereas it affected that conveyed by ERα. The FSH enhancement of hAMH-luc was abolished by estradiol in cells overexpressing ERβ. When both ER were transfected, estradiol inhibited hAMH-luc or had no effect. Estradiol repressed AMH mRNAs in human GC, which express a little more ERα than ERβ mRNA. Conclusions: Our results show that AMH expression can be differentially regulated by estradiol depending on the ER and suggest that its decrease in GC of growing follicles, which mainly express ERβ, and during controlled ovarian hyperstimulation is due to the effect of estradiol.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Endocrine Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Anti-Müllerian Hormone
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Ovary
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Estrogen Receptor
dc.subject.classification
Otras Medicina Básica
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Differential regulation of ovarian anti-müllerian hormone (AMH) by estradiol through α- and β-estrogen receptors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-09T17:48:21Z
dc.journal.volume
97
dc.journal.number
9
dc.journal.pagination
1649-1657
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Grynberg, Michaël. Inserm; Francia
dc.description.fil
Fil: Pierre, Alice. Inserm; Francia
dc.description.fil
Fil: Rey, Rodolfo Alberto. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Leclerc, Arnaud. Inserm; Francia
dc.description.fil
Fil: Arouche, Nassim. Inserm; Francia
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Fil: Hesters, Laetitia. Inserm; Francia
dc.description.fil
Fil: Catteau Jonard, Sophie. Universite Lille; Francia
dc.description.fil
Fil: Frydman, René. Inserm; Francia
dc.description.fil
Fil: Picard, Jean Yves. Inserm; Francia
dc.description.fil
Fil: Fanchin, Renato. Inserm; Francia
dc.description.fil
Fil: Veitia, Reiner. Centre National de la Recherche Scientifique; Francia
dc.description.fil
Fil: Di Clemente, Nathalie. Inserm; Francia
dc.description.fil
Fil: Taieb, Joëlle. Inserm; Francia
dc.journal.title
Journal of Clinical Endocrinology and Metabolism
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1210/jc.2011-3133
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jcem/article/97/9/E1649/2536530
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