Mostrar el registro sencillo del ítem
dc.contributor.author
Trilling, Mirko
dc.contributor.author
Bellora, Nicolás
dc.contributor.author
Rutkowski, Andrzej J.
dc.contributor.author
de Graaf, Miranda
dc.contributor.author
Dickinson, Paul
dc.contributor.author
Robertson, Kevin
dc.contributor.author
Da Costa, Olivia Prazeres
dc.contributor.author
Ghazal, Peter
dc.contributor.author
Friedel, Caroline C.
dc.contributor.author
Albà, M. Mar
dc.contributor.author
Dölken, Lars
dc.date.available
2016-07-29T16:45:43Z
dc.date.issued
2013-09
dc.identifier.citation
Trilling, Mirko; Bellora, Nicolás; Rutkowski, Andrzej J.; de Graaf, Miranda; Dickinson, Paul; et al.; Deciphering the modulation of gene expression by type I and II interferons combining 4sU-tagging, translational arrest and in silico promoter analysis; Oxford University Press; Nucleic Acids Research; 41; 17; 9-2013; 8107-8125
dc.identifier.issn
0305-1048
dc.identifier.uri
http://hdl.handle.net/11336/6809
dc.description.abstract
Interferons (IFN) play a pivotal role in innate immunity, orchestrating a cell-intrinsic anti-pathogenic state and stimulating adaptive immune responses. The complex interplay between the primary response to IFNs and its modulation by positive and negative feedback loops is incompletely understood. Here, we implement the combination of high-resolution gene-expression profiling of nascent RNA with translational inhibition of secondary feedback by cycloheximide. Unexpectedly, this approach revealed a prominent role of negative feedback mechanisms during the immediate (≤60 min) IFNα response. In contrast, a more complex picture involving both negative and positive feedback loops was observed on IFNγ treatment. IFNγ-induced repression of genes associated with regulation of gene expression, cellular development, apoptosis and cell growth resulted from cycloheximide-resistant primary IFNγ signalling. In silico promoter analysis revealed significant overrepresentation of SP1/SP3-binding sites and/or GC-rich stretches. Although signal transducer and activator of transcription 1 (STAT1)-binding sites were not overrepresented, repression was lost in absence of STAT1. Interestingly, basal expression of the majority of these IFNγ-repressed genes was dependent on STAT1 in IFN-naïve fibroblasts. Finally, IFNγ-mediated repression was also found to be evident in primary murine macrophages. IFN-repressed genes include negative regulators of innate and stress response, and their decrease may thus aid the establishment of a signalling perceptive milieu.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Interferon
dc.subject
4su-Tagging
dc.subject
Bioinformatics
dc.subject
Expression
dc.subject.classification
Ciencias de la Información y Bioinformática
dc.subject.classification
Ciencias de la Computación e Información
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.subject.classification
Métodos de Investigación en Bioquímica
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Deciphering the modulation of gene expression by type I and II interferons combining 4sU-tagging, translational arrest and in silico promoter analysis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-07-28T18:33:45Z
dc.identifier.eissn
1362-4962
dc.journal.volume
41
dc.journal.number
17
dc.journal.pagination
8107-8125
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Trilling, Mirko. Universitat Duisburg - Essen; Alemania
dc.description.fil
Fil: Bellora, Nicolás. Parque de Investigación Biomédica de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina
dc.description.fil
Fil: Rutkowski, Andrzej J.. University of Cambridge; Reino Unido
dc.description.fil
Fil: de Graaf, Miranda. University of Cambridge; Reino Unido
dc.description.fil
Fil: Dickinson, Paul. University Of Edinburgh; Reino Unido
dc.description.fil
Fil: Robertson, Kevin. University Of Edinburgh; Reino Unido
dc.description.fil
Fil: Da Costa, Olivia Prazeres. Universitat Technical Zu Munich; Alemania
dc.description.fil
Fil: Ghazal, Peter. University Of Edinburgh; Reino Unido
dc.description.fil
Fil: Friedel, Caroline C.. Ludwig-Maximilians-University Munich; Alemania
dc.description.fil
Fil: Albà, M. Mar. Institució Catalana de Recerca I Estudis Avancats; España. Parque de Investigación Biomédica de Barcelona; España
dc.description.fil
Fil: Dölken, Lars. University of Cambridge; Reino Unido
dc.journal.title
Nucleic Acids Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://nar.oxfordjournals.org/content/41/17/8107
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783172/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/PMC3783172
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkt589
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/nar/gkt589
Archivos asociados