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Artículo

The actin filament network associated to Sertoli cell ectoplasmic specializations

Cavicchia, Juan CarlosIcon ; Foscolo, Mabel RosaIcon ; Ibañez, Jorge ErnestoIcon ; Lillig, Christopher; Capani, FranciscoIcon
Fecha de publicación: 12/2011
Editorial: Instituto de Histología y Embriología
Revista: Biocell
ISSN: 0327-9545
e-ISSN: 1667-5746
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Inmunología

Resumen

Junctional devices in Sertoli cells conform the blood-testis barrier and play a key role in maturation and differentiation of germ cells. The spacial distribution of ectoplasmic specializations of Sertoli cells was studied by β-actin immunolabelling, using laser confocal and transmission electron microscopy. For confocal microscopy, β-actin immunolabelling of ectoplasmic specializations was studied over the background of either prosaposin or glutaredoxin immunolabelling of the Sertoli cytoplasm. Labelling was found near the basal lamina, surrounding early spermatocytes (presumably in leptotene-zygotene) or at one of two levels in the seminiferous epithelium: (1) around deep infoldings of the Sertoli cell cytoplasm, in tubular stages before spermiation, and (2) in the superficial part of the seminiferous epithelium, in tubular stages after or during spermiation. For transmission electron microscopy, β-actin immunolabelling of ectoplasmic specializations was also used. Ectoplasmic specializations were found at two different levels of the seminiferous epithelium. We also used freeze fracture to analyze the characteristics of tubulo-bulbar complexes, a known component of apical ectoplasmic specializations. Also, these different approaches allowed us to study the complex arrangement of the actin cytoskeleton of Sertoli cells branches, which surround germ cells in different stages of the spermatogenic cycle. Our results show a consistent labelling for β-actin before, during and after the release of spermatozoa in the tubular lumen (spermiation) suggesting a significant role of the actin network in spermatic cell differentiation. In conclusion, significant interrelations among the β-actin network, the junctional complexes of the blood-testis barrier and the ectoplasmic specializations were detected at different stages of the seminiferous cycle.
Palabras clave: Ectoplasmic Specializations , Sertoli Cell , Glutaredoxin , Meiosis , Β-Actin , Seminiferous Epithelium
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial 2.5 Unported (CC BY-NC 2.5)
Identificadores
URI: http://hdl.handle.net/11336/67796
URL: http://ref.scielo.org/b8v85v
URL: https://www.mendoza-conicet.gob.ar/portal/biocell/vol/35_3.htm
Colecciones
Articulos(ININCA)
Articulos de INST.DE INVEST.CARDIOLOGICAS (I)
Citación
Cavicchia, Juan Carlos; Foscolo, Mabel Rosa; Ibañez, Jorge Ernesto; Lillig, Christopher; Capani, Francisco; The actin filament network associated to Sertoli cell ectoplasmic specializations; Instituto de Histología y Embriología; Biocell; 35; 3; 12-2011; 81-89
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