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dc.contributor.author
Herrera, Fernando Enrique
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dc.contributor.author
Sevrain, Charlotte M.
dc.contributor.author
Jaffrès, Paul Alain
dc.contributor.author
Couthon, Hélène
dc.contributor.author
Grélard, Axelle
dc.contributor.author
Dufourc, Erick J.
dc.contributor.author
Chantome, Aurélie
dc.contributor.author
Potier Cartereau, Marie
dc.contributor.author
Vandier, Christophe
dc.contributor.author
Bouchet, Ana María
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dc.date.available
2019-01-09T17:33:52Z
dc.date.issued
2017-10
dc.identifier.citation
Herrera, Fernando Enrique; Sevrain, Charlotte M.; Jaffrès, Paul Alain; Couthon, Hélène; Grélard, Axelle; et al.; Singular interaction between an antimetastatic agent and the lipid bilayer: The ohmline case; American Chemical Society; ACS Omega; 2; 10; 10-2017; 6361-6370
dc.identifier.issn
2470-1343
dc.identifier.uri
http://hdl.handle.net/11336/67784
dc.description.abstract
SK3 channels are abnormaly expressed in metastatic cells, and Ohmline (OHM), an ether lipid, has been shown to reduce the activity of SK3 channels and the migration capacity of cancer cells. OHM incorporation into the plasma membrane is proposed to dissociate the protein complex formed between SK3 and Orai1, a potassium and a calcium channel, respectively, and would lead to a modification in the lipid environment of both the proteins. Here, we report the synthesis of deuterated OHM that affords the determination, through solidstate NMR, of its entire partitioning into membranes mimicking the SK3 environment. Use of deuterated lipids affords the demonstration of an OHMinduced membrane disordering, which is dose-dependent and increases with increasing amounts of cholesterol (CHOL). Molecular dynamics simulations comfort the disordering action and show that OHM interacts with the carbonyl and phosphate groups of stearoylphosphatidylcholine and sphingomyelin and to a minor extent with CHOL. OHM is thus proposed to remove the CHOL OH moieties away from their main binding sites, forcing a new rearrangement with other lipid groups. Such an interaction takes its origin at the lipid-water interface, but it propagates toward the entire lipid molecules and leads to a cooperative destabilization of the lipid acyl chains, that is, membrane disordering. The consequences of this reorganization of the lipid phases are discussed in the context of the OHM-induced inhibition of SK3 channels.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ohmline
dc.subject
Sk3
dc.subject
Molecular Dynamics
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Singular interaction between an antimetastatic agent and the lipid bilayer: The ohmline case
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-04T21:37:39Z
dc.journal.volume
2
dc.journal.number
10
dc.journal.pagination
6361-6370
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington
dc.description.fil
Fil: Herrera, Fernando Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina
dc.description.fil
Fil: Sevrain, Charlotte M.. Université de Brest; Francia. Network and Cancer-Canceropole Grand Ouest; Francia
dc.description.fil
Fil: Jaffrès, Paul Alain. Université de Brest; Francia. Network and Cancer-Canceropole Grand Ouest; Francia
dc.description.fil
Fil: Couthon, Hélène. Université de Brest; Francia. Network and Cancer-Canceropole Grand Ouest; Francia
dc.description.fil
Fil: Grélard, Axelle. Universite de Bordeaux; Francia
dc.description.fil
Fil: Dufourc, Erick J.. Universite de Bordeaux; Francia
dc.description.fil
Fil: Chantome, Aurélie. Network and Cancer-Canceropole Grand Ouest; Francia. Université François Rabelais de Tour; Francia
dc.description.fil
Fil: Potier Cartereau, Marie. Network and Cancer-Canceropole Grand Ouest; Francia. Université François Rabelais de Tour; Francia
dc.description.fil
Fil: Vandier, Christophe. Network and Cancer-Canceropole Grand Ouest; Francia. Université François Rabelais de Tour; Francia
dc.description.fil
Fil: Bouchet, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Network and Cancer-Canceropole Grand Ouest; Francia. Université François Rabelais de Tour; Francia
dc.journal.title
ACS Omega
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1021/acsomega.7b00936
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acsomega.7b00936
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