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Artículo

Recording gamma band oscillations in pedunculopontine nucleus neurons

Urbano Suarez, Francisco JoseIcon ; Luster, Brennon; D´Onofrio, Stasia; Mahaffey, Susan; Garcia Rill, Edgar
Fecha de publicación: 09/2016
Editorial: JoVE
Revista: Journal of Visualized Experiments
ISSN: 1940-087X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Inmunología

Resumen

Synaptic efferents from the PPN are known to modulate the neuronal activity of several intralaminar thalamic regions (e.g., the centrolateral/ parafascicular; Cl/Pf nucleus). The activation of either the PPN or Cl/Pf nuclei in vivo has been described to induce the arousal of the animal and an increment in gamma band activity in the cortical electroencephalogram (EEG). The cellular mechanisms for the generation of gamma band oscillations in Reticular Activating System (RAS) neurons are the same as those found to generate gamma band oscillations in other brains nuclei. During current-clamp recordings of PPN neurons (from parasagittal slices from 9 - 25 day-old rats), the use of depolarizing square steps rapidly activated voltage-dependent potassium channels that prevented PPN neurons from being depolarized beyond -25 mV. Injecting 1 - 2 sec long depolarizing current ramps gradually depolarized PPN membrane potential resting values towards 0 mV. However, injecting depolarizing square pulses generated gamma-band oscillations of membrane potential that showed to be smaller in amplitude compared to the oscillations generated by ramps. All experiments were performed in the presence of voltage-gated sodium channels and fast synaptic receptors blockers. It has been shown that the activation of high-threshold voltage-dependent calcium channels underlie gamma-band oscillatory activity in PPN neurons. Specific methodological and pharmacological interventions are described here, providing the necessary tools to induce and sustain PPN subthreshold gamma band oscillation in vitro.
Palabras clave: Arousal , Ca2+Channels , Current Ramps , Gamma Oscillations , Issue 115 , N-Type , Neuroscience , P/Q-Type
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/67694
URL: https://www.jove.com/video/54685/recording-gamma-band-oscillations-in-pedunculop
DOI: http://dx.doi.org/10.3791/54685
Colecciones
Articulos(IFIBYNE)
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Citación
Urbano Suarez, Francisco Jose; Luster, Brennon; D´Onofrio, Stasia; Mahaffey, Susan; Garcia Rill, Edgar; Recording gamma band oscillations in pedunculopontine nucleus neurons; JoVE; Journal of Visualized Experiments; 2016; 115; 9-2016; 1-10
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