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Artículo

Alternative infectious entry pathways for dengue virus serotypes into mammalian cells

Acosta, Eliana GiselaIcon ; Castilla, Viviana; Damonte, Elsa BeatrizIcon
Fecha de publicación: 10/2009
Editorial: Wiley Blackwell Publishing, Inc
Revista: Cellular Microbiology (print)
ISSN: 1462-5814
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

The entry of two dengue virus (DENV) serotypes into Vero cells was analysed using biochemical inhibitors, dominant negative mutants of cellular proteins involved in endocytic pathways, fluorescence microscopy and infectivity determinations. By treatment with dansylcadaverine and chlorpromazine and overexpression of a dominant negative form of the Eps15 protein, a clathrin-mediated endocytosis for productive DENV-1 internalization into Vero cells was demonstrated whereas the infectious entry of DENV-2 in the same cell system was independent of clathrin. Treatment with the inhibitors nystatin and methyl-β-cyclodextrin, as well as transfection of Vero cells with dominant negative caveolin-1, had no effect on DENV-2 virus infection. It was also shown, by using the K44A mutant and the inhibitor dynasore, that dynamin was required for DENV-2 entry. Consequently, the infectious entry of DENV-2 into Vero cells occurs by a non-classical endocytic pathway independent of clathrin, caveolae and lipid rafts, but dependent on dynamin. By contrast, DENV-2 entry into A549 cells was clathrin-dependent, as previously reported in HeLa, C6/36 and BS-C-1 cells. Our results conclusively show, for the first time, a differential mode of infective entry for DENV-1 and DENV-2 into a common host cell, Vero cells, as well as alternative entry pathways for a given serotype, DENV-2, into different types of cells. © 2009 Blackwell Publishing Ltd.
Palabras clave: Dengue Virus , Virus Entry , Mammalian Cells
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/67648
DOI: https://dx.doi.org/10.1111/j.1462-5822.2009.01345.x
URL: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1462-5822.2009.01345.x
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Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Acosta, Eliana Gisela; Castilla, Viviana; Damonte, Elsa Beatriz; Alternative infectious entry pathways for dengue virus serotypes into mammalian cells; Wiley Blackwell Publishing, Inc; Cellular Microbiology (print); 11; 10; 10-2009; 1533-1549
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