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dc.contributor.author
Metaxas, Athanasios  
dc.contributor.author
Bailey, Alexis  
dc.contributor.author
Barbano, María Flavia  
dc.contributor.author
Galeote, Lola  
dc.contributor.author
Maldonado, Rafael  
dc.contributor.author
Kitchen, Ian  
dc.date.available
2019-01-07T19:11:07Z  
dc.date.issued
2010-10  
dc.identifier.citation
Metaxas, Athanasios; Bailey, Alexis; Barbano, María Flavia; Galeote, Lola; Maldonado, Rafael; et al.; Differential region-specific regulation of α4β2* nAChRs by self-administered and non-contingent nicotine in C57BL/6J mice; Wiley Blackwell Publishing, Inc; Addiction Biology; 15; 4; 10-2010; 464-479  
dc.identifier.issn
1355-6215  
dc.identifier.uri
http://hdl.handle.net/11336/67583  
dc.description.abstract
Neuronal nAChR upregulation is the hallmark of chronic nicotine exposure. Neuroplasticity to abused drugs, however, depends on whether their administration is forced by the experimenter or is under the control of the experimental animal. Neuroadaptation to chronic nicotine self-administration was examined with a yoked-control paradigm, using nose-poking as the operating procedure. Freely moving C57BL/6J mice that responded for 0.03 mg/kg/infusion of intravenous nicotine under a continuous schedule of reinforcement (FR-1), had control over the rate and amount of drug intake that a yoked littermate passively received (n = 11). The impact of response dependency on neurobiological changes in nicotinic and dopaminergic systems was subsequently assessed using quantitative autoradiography. Cytisine-sensitive [ 125I]epibatidine binding, [3H]SCH23390, [ 3H]raclopride and [3H]mazindol were used to label nAChRs with α4β2* subtype properties, D1 and D2 dopaminergic receptors, and dopamine transporters, respectively. During a period of 12 days, self-administration was reliably initiated and maintained in animals receiving response-contingent nicotine. Region specific changes in the density of α4β2* nAChRs were found to be dependent on the contingency of nicotine treatment. Higher levels of α4β2* receptor binding were observed in the dorsal lateral geniculate nucleus and the ventral tegmental area of self-administering mice, compared to non-contingent animals. Moreover, response-independent increases in D2 binding were observed following chronic nicotine administration. No change in D1 and DAT binding was observed among groups. These findings indicate regional specific alterations in the regulation of the nicotinic cholinergic system following contingent and non-contingent nicotine exposure, and underline the importance of response dependency on the development of nicotine addiction.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley Blackwell Publishing, Inc  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Autoradiography  
dc.subject
Mouse  
dc.subject
Nicotine  
dc.subject
Operant Contingency  
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Self-Administration  
dc.subject.classification
Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Differential region-specific regulation of α4β2* nAChRs by self-administered and non-contingent nicotine in C57BL/6J mice  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-01-07T13:31:31Z  
dc.journal.volume
15  
dc.journal.number
4  
dc.journal.pagination
464-479  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Metaxas, Athanasios. University of Surrey; Reino Unido  
dc.description.fil
Fil: Bailey, Alexis. University of Surrey; Reino Unido  
dc.description.fil
Fil: Barbano, María Flavia. Universitat Pompeu Fabra; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Galeote, Lola. Universitat Pompeu Fabra; España  
dc.description.fil
Fil: Maldonado, Rafael. Universitat Pompeu Fabra; España  
dc.description.fil
Fil: Kitchen, Ian. University of Surrey; Reino Unido  
dc.journal.title
Addiction Biology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1369-1600.2010.00246.x  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1369-1600.2010.00246.x