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dc.contributor.author
Bracamonte, Angel Guillermo
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dc.contributor.author
Veglia, Alicia Viviana
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dc.date.available
2019-01-04T12:40:58Z
dc.date.issued
2011-01
dc.identifier.citation
Bracamonte, Angel Guillermo; Veglia, Alicia Viviana; Spectrofluorimetric determination of serotonin and 5-hydroxyindoleacetic acid in urine with different cyclodextrin media; Elsevier Science; Talanta; 83; 3; 1-2011; 1006-1013
dc.identifier.issn
0039-9140
dc.identifier.uri
http://hdl.handle.net/11336/67372
dc.description.abstract
Alternative and sensitive spectrofluorimetric methods for the determination of hydroxyindoles, such as serotonin (5HT) and 5-hydroxyindoleacetic acid (5HIA), were developed on the basis of supramolecular interaction with cyclodextrin (CD) nanocavities (βCD and hydroxypropyl-βCD, HPCD) at different pH values. Both substrates and receptors have acidic protons, therefore the interactions produced in different systems were considered. The effects of neutral CD at pH 2.00 and 6.994, and of anionic CD at pH 13.00 on the specific acid-base species of the compounds at each pH were determined. In all the conditions studied, the fluorescence of the substrates in the presence of CD increased. The association constants (KA, mol-1 L) between the substrates and CD were determined (30-300) and interpreted. A zero-crossing first-derivative spectrofluorimetric method with and without HPCD was developed for the simultaneous determination of 5HT and 5HIA. The limits of detection (LD, ng mL-1) for the best conditions were 0.37 for 5HT and 0.50 for 5HIA at pH 2.00 with HPCD. These LD proved to be better than others reported. The applicability of the direct and derivative spectrofluorimetric methods to urine samples was demonstrated with good recoveries 92-110% and R.S.D. 1-10%. © 2010 Elsevier B.V.All rights reserved.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Acid-Base Equilibrium
dc.subject
Cyclodextrin Nanocavities
dc.subject
Fluorescence
dc.subject
Hydroxyindoles
dc.subject
Sensitive Determination
dc.subject
Supramolecular Systems
dc.subject.classification
Otras Ciencias Químicas
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Spectrofluorimetric determination of serotonin and 5-hydroxyindoleacetic acid in urine with different cyclodextrin media
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-02T19:11:02Z
dc.journal.volume
83
dc.journal.number
3
dc.journal.pagination
1006-1013
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.journal.title
Talanta
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0039914010008738
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.talanta.2010.11.013
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