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dc.contributor.author
Vico, Raquel Viviana
dc.contributor.author
Voskuhl, Jens
dc.contributor.author
Ravoo, Bart Jan
dc.date.available
2019-01-04T12:40:38Z
dc.date.issued
2011-02
dc.identifier.citation
Vico, Raquel Viviana; Voskuhl, Jens; Ravoo, Bart Jan; Multivalent interaction of cyclodextrin vesicles, carbohydrate guests, and lectins: A kinetic investigation; American Chemical Society; Langmuir; 27; 4; 2-2011; 1391-1397
dc.identifier.issn
0743-7463
dc.identifier.uri
http://hdl.handle.net/11336/67369
dc.description.abstract
An artificial glycocalix self-assembles when unilamellar bilayer vesicles of amphiphilic β-cyclodextrins are decorated with maltose-and lactose-adamantane conjugates by host-guest interactions. The maltose-decorated vesicles aggregate in the presence of lectin concanavalin A whereas the lactose-decorated vesicles aggregate in the presence of lectin peanut agglutinin. The kinetics of the orthogonal multivalent interfacial interactions present in this ternary system of vesicles, carbohydrates, and lectins were studied by time-dependent measurements of the optical density at 400 nm. The average vesicle and vesicle aggregate sizes were monitored by dynamic light scattering. The aggregation process was evaluated as a function of lectin concentration, vesicle concentration, and surface coverage of the vesicles by the carbohydrate-adamantane conjugates. The initial rate of vesicle aggregation scales linearly with the lectin as well as the cyclodextrin vesicle concentration. Furthermore, each lectin requires a characteristic critical density of carbohydrates at the vesicle surface. These observations allow a prediction of the response of the ternary supramolecular system at different concentrations of its components. Also, the effective binding site separation in a multivalent receptor such as a multiple binding site protein can be accurately determined. This methodology can be extended to multivalent noncovalent interactions in other ligand-receptor systems at interfaces. © 2010 American Chemical Society.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cyclodextrin
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Carbohydrates
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Lectins
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Multivalency
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Multivalent interaction of cyclodextrin vesicles, carbohydrate guests, and lectins: A kinetic investigation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-02T19:10:53Z
dc.journal.volume
27
dc.journal.number
4
dc.journal.pagination
1391-1397
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Vico, Raquel Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Voskuhl, Jens. Westfalische Wilhelms Universitat; Alemania
dc.description.fil
Fil: Ravoo, Bart Jan. Westfalische Wilhelms Universitat; Alemania
dc.journal.title
Langmuir
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/la1038975
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/la1038975
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