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dc.contributor.author
Cuestas, María Luján
dc.contributor.author
Mathet, Veronica Lidia
dc.contributor.author
Oubiña, Jose Raul
dc.date.available
2019-01-03T17:53:07Z
dc.date.issued
2012-10
dc.identifier.citation
Cuestas, María Luján; Mathet, Veronica Lidia; Oubiña, Jose Raul; Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants; Wiley Blackwell Publishing, Inc; Journal Of Viral Hepatitis.; 19; 10; 10-2012; 754-756
dc.identifier.issn
1352-0504
dc.identifier.uri
http://hdl.handle.net/11336/67305
dc.description.abstract
PCR detection of viral genomes has provided new insights into viral diagnosis. Nowadays, it is the most frequently used nucleic acid testing (qualitative and quantitative) technique. The aim of this study was to analyse the major circulating hepatitis B virus (HBV) variants PCR-amplified by three sets of primers in a patient infected with genotype E. The HBV S/Pol overlapping genomic region was amplified from the serum of an infected child using three primer sets previously described. Sequence analysis corresponding to the HBV S/Pol region revealed the presence of different viral populations depending on the set of primers used. D144A S-escape mutant was detected with two of the primer sets, while the rtL217R mutant within the Pol - conferring resistance to Adefovir - could be picked up with a different pair of primer sets. This study undoubtedly implies that the description of viral polymorphisms should be stated together with the sequence of the primers used for PCR amplification when studies of escape and/or antiviral-resistant HBV mutants are carried out.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Hepatitis B Virus
dc.subject
Primer Sets
dc.subject
Variants
dc.subject.classification
Inmunología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-02T19:51:24Z
dc.journal.volume
19
dc.journal.number
10
dc.journal.pagination
754-756
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Cuestas, María Luján. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Mathet, Veronica Lidia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Oubiña, Jose Raul. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal Of Viral Hepatitis.
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1365-2893.2012.01614.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2893.2012.01614.x


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