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dc.contributor.author Cuestas, María Luján
dc.contributor.author Mathet, Veronica Lidia
dc.contributor.author Oubiña, Jose Raul
dc.date.available 2019-01-03T17:53:07Z
dc.date.issued 2012-10
dc.identifier.citation Cuestas, María Luján; Mathet, Veronica Lidia; Oubiña, Jose Raul; Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants; Wiley Blackwell Publishing, Inc; Journal Of Viral Hepatitis.; 19; 10; 10-2012; 754-756
dc.identifier.issn 1352-0504
dc.identifier.uri http://hdl.handle.net/11336/67305
dc.description.abstract PCR detection of viral genomes has provided new insights into viral diagnosis. Nowadays, it is the most frequently used nucleic acid testing (qualitative and quantitative) technique. The aim of this study was to analyse the major circulating hepatitis B virus (HBV) variants PCR-amplified by three sets of primers in a patient infected with genotype E. The HBV S/Pol overlapping genomic region was amplified from the serum of an infected child using three primer sets previously described. Sequence analysis corresponding to the HBV S/Pol region revealed the presence of different viral populations depending on the set of primers used. D144A S-escape mutant was detected with two of the primer sets, while the rtL217R mutant within the Pol - conferring resistance to Adefovir - could be picked up with a different pair of primer sets. This study undoubtedly implies that the description of viral polymorphisms should be stated together with the sequence of the primers used for PCR amplification when studies of escape and/or antiviral-resistant HBV mutants are carried out.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley Blackwell Publishing, Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject HEPATITIS B VIRUS
dc.subject PRIMER SETS
dc.subject VARIANTS
dc.subject.classification Inmunología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2019-01-02T19:51:24Z
dc.journal.volume 19
dc.journal.number 10
dc.journal.pagination 754-756
dc.journal.pais Reino Unido
dc.journal.ciudad Londres
dc.description.fil Fil: Cuestas, María Luján. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Mathet, Veronica Lidia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Oubiña, Jose Raul. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Journal Of Viral Hepatitis.
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1365-2893.2012.01614.x
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2893.2012.01614.x
dc.conicet.fuente unificacion


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    Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA

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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)