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dc.contributor.author
Pérez Pérez, Antonio
dc.contributor.author
Maymo, Julieta Lorena
dc.contributor.author
Gambino, Yésica Paola
dc.contributor.author
Dueñas, José L.
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Goberna, Raimundo
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Varone, Cecilia Laura
dc.contributor.author
Sánchez Margalet, Víctor
dc.date.available
2019-01-03T13:13:11Z
dc.date.issued
2009-06
dc.identifier.citation
Pérez Pérez, Antonio; Maymo, Julieta Lorena; Gambino, Yésica Paola; Dueñas, José L.; Goberna, Raimundo; et al.; Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells; Society for the Study of Reproduction; Biology of Reproduction; 81; 5; 6-2009; 826-832
dc.identifier.issn
0006-3363
dc.identifier.uri
http://hdl.handle.net/11336/67270
dc.description.abstract
Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Society for the Study of Reproduction
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Leptin
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Leptin Receptor
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Mechanisms of Hormone Action
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Placenta
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Trophoblast
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Otras Ciencias Biológicas
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-02T19:13:34Z
dc.journal.volume
81
dc.journal.number
5
dc.journal.pagination
826-832
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Madison
dc.description.fil
Fil: Pérez Pérez, Antonio. Universidad de Sevilla; España
dc.description.fil
Fil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Dueñas, José L.. Universidad de Sevilla; España
dc.description.fil
Fil: Goberna, Raimundo. Universidad de Sevilla; España
dc.description.fil
Fil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Sánchez Margalet, Víctor. Universidad de Sevilla; España
dc.journal.title
Biology of Reproduction
dc.relation.isreferencedin
info:eu-repo/semantics/reference/url/http://hdl.handle.net/11336/11683
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1095/biolreprod.109.076513
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/81/5/826/2557758


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