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dc.contributor.author Pérez Pérez, Antonio
dc.contributor.author Maymo, Julieta Lorena
dc.contributor.author Gambino, Yésica Paola
dc.contributor.author Dueñas, José L.
dc.contributor.author Goberna, Raimundo
dc.contributor.author Varone, Cecilia Laura
dc.contributor.author Sánchez Margalet, Víctor
dc.date.available 2019-01-03T13:13:11Z
dc.date.issued 2009-06
dc.identifier.citation Pérez Pérez, Antonio; Maymo, Julieta Lorena; Gambino, Yésica Paola; Dueñas, José L.; Goberna, Raimundo; et al.; Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells; Society for the Study of Reproduction; Biology of Reproduction; 81; 5; 6-2009; 826-832
dc.identifier.issn 0006-3363
dc.identifier.uri http://hdl.handle.net/11336/67270
dc.description.abstract Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc.
dc.format application/pdf
dc.language.iso eng
dc.publisher Society for the Study of Reproduction
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject LEPTIN
dc.subject LEPTIN RECEPTOR
dc.subject MECHANISMS OF HORMONE ACTION
dc.subject PLACENTA
dc.subject TROPHOBLAST
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2019-01-02T19:13:34Z
dc.journal.volume 81
dc.journal.number 5
dc.journal.pagination 826-832
dc.journal.pais Estados Unidos
dc.journal.ciudad Madison
dc.description.fil Fil: Pérez Pérez, Antonio. Universidad de Sevilla; España
dc.description.fil Fil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil Fil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil Fil: Dueñas, José L.. Universidad de Sevilla; España
dc.description.fil Fil: Goberna, Raimundo. Universidad de Sevilla; España
dc.description.fil Fil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil Fil: Sánchez Margalet, Víctor. Universidad de Sevilla; España
dc.journal.title Biology of Reproduction
dc.relation.isreferencedin info:eu-repo/semantics/reference/url/http://hdl.handle.net/11336/11683
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1095/biolreprod.109.076513
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/81/5/826/2557758
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)