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dc.contributor.author Zago, Valeria
dc.contributor.author Miksztowicz, Verónica Julieta
dc.contributor.author Cacciagiú, Leonardo D.
dc.contributor.author Basilio, Francisco
dc.contributor.author Berg, Gabriela Alicia
dc.contributor.author Schreier, Laura Ester
dc.date.available 2019-01-02T22:20:14Z
dc.date.issued 2012-09
dc.identifier.citation Zago, Valeria; Miksztowicz, Verónica Julieta; Cacciagiú, Leonardo D.; Basilio, Francisco; Berg, Gabriela Alicia; et al.; High density lipoprotein is an inappropiate substrate for hepatic lipase in postmenopausal women; Elsevier Science; Clinica Chimica Acta; 414; 9-2012; 142-145
dc.identifier.issn 0009-8981
dc.identifier.uri http://hdl.handle.net/11336/67266
dc.description.abstract Background HDL antiatherogenic effects would not only depend on its concentration but also on its biological quality. Hepatic lipase (HL) action on HDL acts in one of the last steps of reverse cholesterol transport. Cardiovascular risk increases after menopause, however HDL does not decrease even when HL is increased. We evaluated HDL capacity as a substrate of HL in healthy postmenopausal women (PMW). Methods We studied 20 PMW (51–60 y) and 20 premenopausal (PreMW) (26–40 y). In fasting serum, lipid–lipoprotein profile and HDL composition were assessed. Optimal assay conditions for HDL/HL ex vivo incubation were established. Increasing HDL–triglyceride concentrations (0.015 to 0.20 mmol/l) were incubated with post-heparin plasma obtained from a single healthy donor as a source of HL. Free fatty acids were measured and kinetic parameters calculated: Km(app), inverse to enzyme affinity, and Vmax. Results HDL composition in PMW exhibits triglyceride enrichment (p < 0.001). Kinetic analysis revealed higher Km(app) in PMW [130 (40–380) vs 45 (20–91) mmol/l, p < 0.0001)] correlating directly with HDL–triglycerides (r = 0.7, p = 0.0001). Catalytic efficiency, Vmax/Km(app) was reduced when compared to controls (p = 0.0001). Conclusion Triglyceride-enriched HDL from PMW constitutes a poor substrate for HL suggesting that this particle may not exert efficiently its antiatherogenic function, regardless of plasma concentration.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Science
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject HDL FUNCTIONALITY
dc.subject HEPATIC LIPASE
dc.subject HIGH DENSITY LIPOPROTEIN
dc.subject KINETICS
dc.subject POSTMENOPAUSE
dc.subject.classification Salud Ocupacional
dc.subject.classification Ciencias de la Salud
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title High density lipoprotein is an inappropiate substrate for hepatic lipase in postmenopausal women
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2019-01-02T19:55:39Z
dc.journal.volume 414
dc.journal.pagination 142-145
dc.journal.pais Países Bajos
dc.journal.ciudad Amsterdam
dc.description.fil Fil: Zago, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
dc.description.fil Fil: Miksztowicz, Verónica Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
dc.description.fil Fil: Cacciagiú, Leonardo D.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
dc.description.fil Fil: Basilio, Francisco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
dc.description.fil Fil: Berg, Gabriela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
dc.description.fil Fil: Schreier, Laura Ester. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
dc.journal.title Clinica Chimica Acta
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.cca.2012.08.026
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009898112004317
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)