2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor

A new synthesis of 2′-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5′-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor α protein (ER-α), and transfection experiments in MCF-7 cell line are also presented. © 2010 Elsevier Ltd. All rights reserved.

Palabras clave: 2″-C-Methyluridine , Estrogen Receptor Alpha , Modified Ribozyme

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

Identificadores

URI: http://hdl.handle.net/11336/67264

DOI: https://dx.doi.org/10.1016/j.bmcl.2010.03.060

URL: https://linkinghub.elsevier.com/retrieve/pii/S0960894X10003811

Colecciones

Articulos(INGEBI)
Articulos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"

Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY

Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL

Citación

Pontiggia, Rodrigo Martin; Pontiggia, Osvaldo Alejandro; Simian, Marina; Montserrat, Javier Marcelo; Engels, Joachim W.; et al.; 2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor; Pergamon-Elsevier Science Ltd; Biorganic and Medicinal Chemistry Letters; 20; 9; 5-2010; 2806-2808

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