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Artículo

Expression of M Protein from LP02/C Equine Arteritis Virus Inhibits Growth of Escherichia Coli M15-pQE30 System

Metz, German ErnestoIcon ; Abeyá, María MercedesIcon ; Serena, Maria SoledadIcon ; Panei, Carlos JavierIcon ; Diaz, SilvinaIcon ; Echeverria, Maria GabrielaIcon
Fecha de publicación: 05/2017
Editorial: Open Acces text
Revista: Virology: Research and Reviews
ISSN: 2514-4138
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Our objective was to obtain Equine Arteritis Virus M protein in prokaryotic system to test it as immunogen. LP02/C Equine Arteritis Virus cDNA was used astemplate to obtain and clone this protein. Equine Arteritis M protein was cloned in the expression vector pQE30 and the recombinant plasmid pQE30/M wastransformed in Escherichia Coli M15 cells. The OD600 values of the IPTG-induced M15-pQE30/M culture showed an inhibition of the kinetics growth comparedwith the non-induced M15-pQE30/M and positive M15-pQE40/DHFR cultures. Several factors such as growth temperature, IPTG concentration and differentinductors were analyzed but any of them showed an improvement in protein expression. Instead of E. coli M15strain, a new strain (E. coli BL21) was used andtransformed with the pQE30/M. This resolved in part the growth inhibition observed in E. coli M15 cells, but no the recovery yield of the protein. So, as all geneproducts that affect cells kinetics growth are considered to be toxic, we argue that the lower yields in M protein recovery could be attributed to an associated toxicityof EAV-M protein from LP02/C strain in this expression system.
Palabras clave: Equine Arteritis Virus , Lp02/C Strain , M Protein
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/66956
DOI: https://dx.doi.org/10.15761/VRR.1000111
URL: https://www.oatext.com/Expression-of-M-Protein-from-LP02C-Equine-Arteritis-Virus
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(IGEVET)
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Citación
Metz, German Ernesto; Abeyá, María Mercedes; Serena, Maria Soledad; Panei, Carlos Javier; Diaz, Silvina; et al.; Expression of M Protein from LP02/C Equine Arteritis Virus Inhibits Growth of Escherichia Coli M15-pQE30 System; Open Acces text; Virology: Research and Reviews; 1; 2; 5-2017; 1-5
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