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dc.contributor.author
Perez Perez, Antonio
dc.contributor.author
Toro, Ayelen Rayen
dc.contributor.author
Vilarino Garcia,Teresa
dc.contributor.author
Guadix, Pilar
dc.contributor.author
Maymo, Julieta Lorena
dc.contributor.author
Dueñas, Jose
dc.contributor.author
Varone, Cecilia Laura
dc.contributor.author
Sanchez Margalet, Victor
dc.date.available
2018-12-18T20:34:20Z
dc.date.issued
2016-06
dc.identifier.citation
Perez Perez, Antonio; Toro, Ayelen Rayen; Vilarino Garcia,Teresa; Guadix, Pilar; Maymo, Julieta Lorena; et al.; Leptin reduces apoptosis triggered by high temperature in human placental villous explants: The role of the p53 pathway; W B Saunders Co Ltd; Placenta; 42; 6-2016; 106-113
dc.identifier.issn
0143-4004
dc.identifier.uri
http://hdl.handle.net/11336/66706
dc.description.abstract
Maternal fever is common during pregnancy and has for many years been suspected to harm the developing fetus. Whether increased maternal temperature produces exaggerated apoptosis in trophoblast cells remains unclear. Since p53 is a critical regulator of apoptosis we hypothesized that increased temperature in placenta produces abnormal expression of proteins in the p53 pathway and finally caspase-3 activation. Moreover, leptin, produced by placenta, is known to promote the proliferation and survival of trophoblastic cells. Thus, we aimed to study the possible role of leptin preventing apoptosis triggered by high temperature, as well as the molecular mechanisms underlying this effect. Fresh placental tissue was collected from normal pregnancies. Explants of placental villi were exposed to 37°C, 40°C and 42°C during 3 h in the presence or absence of 10 nM leptin in DMEM-F12 medium. Western blotting and qRT-PCR was performed to analyze the expression of p53 and downstream effector, P53AIP1, Mdm2, p21, BAX and BCL-2 as well as the activated cleaved form of caspase-3 and the fragment of cytokeratin-18 (CK-18) cleaved at Asp396 (neoepitope M30). Phosphorylation of the Ser 46 residue on p53, the expression of P53AIP1, Mdm2, p21, as well as caspase-3 and CK-18 were significantly increased in explants at 40°C and 42°C. Conversely, these effects were significantly attenuated by leptin 10 nM at both 40°C and 42°C. The BCL2/BAX ratio was also significantly decreased in explants at 40°C and 42°C compared with explants incubated at 37°C, which was prevented by leptin stimulation. These data illustrate the potential role of leptin for reducing apoptosis in trophoblast explants, including trophoblastic cells, triggered by high temperature, by preventing the activation of p53 signaling.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
W B Saunders Co Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Apoptosis
dc.subject
Ck-18(M30)
dc.subject
Leptin
dc.subject
P53
dc.subject
Placenta
dc.subject
Temperature
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Leptin reduces apoptosis triggered by high temperature in human placental villous explants: The role of the p53 pathway
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-08-30T15:11:21Z
dc.journal.volume
42
dc.journal.pagination
106-113
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Perez Perez, Antonio. Universidad de Sevilla; España
dc.description.fil
Fil: Toro, Ayelen Rayen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Vilarino Garcia,Teresa. Universidad de Sevilla; España
dc.description.fil
Fil: Guadix, Pilar. Universidad de Sevilla; España
dc.description.fil
Fil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Dueñas, Jose. Universidad de Sevilla; España
dc.description.fil
Fil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Sanchez Margalet, Victor. Universidad de Sevilla; España
dc.journal.title
Placenta
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.placenta.2016.03.009
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0143400416300455


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