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dc.contributor.author Acosta, Cristian Gabriel
dc.contributor.author Fabrega, Andres Rolando
dc.contributor.author Masco, Daniel Hugo
dc.contributor.author Lopez, Hector Salvador
dc.date.available 2018-12-18T16:40:18Z
dc.date.issued 2001-11
dc.identifier.citation Acosta, Cristian Gabriel; Fabrega, Andres Rolando; Masco, Daniel Hugo; Lopez, Hector Salvador; A Sensory Neuron Subpopulation with Unique Sequential Survival Dependence on Nerve Growth Factor and Basic Fibroblast Growth Factor during Development; Society for Neuroscience; Journal of Neuroscience; 21; 22; 11-2001; 8873-8885
dc.identifier.issn 0270-6474
dc.identifier.uri http://hdl.handle.net/11336/66663
dc.description.abstract We characterized a subpopulation of dorsal root ganglion (DRG) sensory neurons that were previously identified as preferential targets of enkephalins. This group, termed P-neurons after their “pear” shape, sequentially required nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) for survival in vitroduring different developmental stages. Embryonic P-neurons required NGF, but not bFGF. NGF continued to promote their survival, although less potently, up to postnatal day 2 (P2). Conversely, at P5, they needed bFGF but not NGF, with either factor having similar effects at P2. This trophic switch was unique to that DRG neuronal group. In addition, neither neurotrophin-3 (NT-3) nor brain-derived neurotrophic factor influenced their survival during embryonic and postnatal stages, respectively. The expression of NGF (Trk-A) and bFGF (flg) receptors paralleled the switch in trophic requirement. No single P-neuron appeared to coexpress bothTrk-A and flg. In contrast, all of them coexpressed flg and substance P, providing a specific marker of these cells. Immunosuppression of bFGF in newborn animals greatly reduced their number, suggesting that the factor was requiredin vivo. bFGF was present in the DRG and spinal cord, as well as in skeletal muscle, the peripheral projection site of P-neurons, as revealed by tracer DiIC183. The lack of requirement of NT-3 for survival and immunoreactivity for the neurofilament of 200 kDa distinguished them from muscle proprioceptors, suggesting that they are likely to be unmyelinated muscle fibers. Collectively, their properties indicate that P-neurons constitute a distinct subpopulation of sensory neurons for which the function may be modulated by enkephalins.
dc.format application/pdf
dc.language.iso eng
dc.publisher Society for Neuroscience
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject DORSAL ROOT GANGLION
dc.subject NGF
dc.subject BFGF
dc.subject SWITCH
dc.subject.classification Inmunología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title A Sensory Neuron Subpopulation with Unique Sequential Survival Dependence on Nerve Growth Factor and Basic Fibroblast Growth Factor during Development
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-11-12T13:46:51Z
dc.journal.volume 21
dc.journal.number 22
dc.journal.pagination 8873-8885
dc.journal.pais Estados Unidos
dc.journal.ciudad Washington
dc.description.fil Fil: Acosta, Cristian Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.description.fil Fil: Fabrega, Andres Rolando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.description.fil Fil: Masco, Daniel Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Biología Celular; Argentina
dc.description.fil Fil: Lopez, Hector Salvador. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.journal.title Journal of Neuroscience
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/21/22/8873
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1523/JNEUROSCI.21-22-08873.2001
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)